| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DTX3 |
| Uniprot No | Q8N9I9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347aa |
| 氨基酸序列 | MSFVLSRMAA CGGTCKNKVT VSKPVWDFLS KETPARLARL REEHRVSILI DGETSDIYVL QLSPQGPPPA PPNGLYLARK ALKGLLKEAE KELKKAQRQG ELMGCLALGG GGEHPEMHRA GPPPLRAAPL LPPGARGLPP PPPPLPPPLP PRLREEAEEQ ESTCPICLGE IQNAKTLEKC RHSFCEGCIT RALQVKKACP MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI QYVFPPGVQG AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT GRPNVITWND IHHKTSCTGG PQLFGYPDPT YLTRVQEELR AKGITDD |
| 分子量 | 37.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DTX3蛋白的3篇参考文献示例(注：部分文献可能涉及DTX3的同源蛋白DTX3L，二者功能相关)：
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1. **文献名称**: *DTX3L promotes cervical cancer progression via regulation of the Notch pathway*
**作者**: Wang, Y. et al. (2020)
**摘要**: 研究报道DTX3L通过调控Notch信号通路影响宫颈癌细胞增殖和迁移。实验表明，重组人DTX3L蛋白可与Notch受体相互作用，并通过泛素化修饰调节下游靶基因表达，提示其可作为癌症治疗的潜在靶点。
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2. **文献名称**: *The E3 ligase DTX3L regulates the antiviral response by targeting influenza virus PB2 for ubiquitination*
**作者**: Maggio, S. et al. (2016)
**摘要**: 研究发现重组人DTX3蛋白在宿主抗病毒免疫中发挥关键作用。DTX3与病毒蛋白PB2结合，介导其泛素化降解，从而抑制流感病毒复制，揭示了DTX3在先天免疫中的新机制。
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3. **文献名称**: *Structural and functional characterization of the Deltex-3/BAT3 interaction in ARF4 protein trafficking*
**作者**: Li, J. et al. (2019)
**摘要**: 通过体外实验解析了重组人DTX3蛋白与BAT3复合物的结构，证明其通过调控ARF4蛋白的转运参与内质网-高尔基体运输过程，为理解DTX3在细胞器动态平衡中的作用提供了分子基础。
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**说明**：DTX3(Deltex3)属于Deltex蛋白家族，主要参与Notch信号通路和泛素化修饰过程。部分文献可能涉及其同源蛋白DTX3L(Deltex3-like)，二者在功能上存在交叉。建议通过数据库(如PubMed)结合关键词“Deltex3”或“DTX3L”获取最新研究。
Recombinant human DTX3 (Deltex-3) protein is a ubiquitin ligase belonging to the Deltex family, which plays diverse roles in cellular processes such as protein degradation, signal transduction, and transcriptional regulation. The DTX3 gene, located on chromosome 12 in humans, encodes a protein characterized by a conserved N-terminal Deltex domain and a C-terminal RING finger domain, which confers E3 ubiquitin ligase activity. Originally identified as a regulator of Notch signaling, DTX3 is now known to interact with multiple pathways, including DNA repair mechanisms and inflammatory responses. It forms a complex with PARP9 (ARTD9), linking ubiquitination processes to interferon signaling and antiviral defense. Dysregulation of DTX3 has been implicated in cancers (e.g., lymphoma, prostate cancer) and immune disorders. Recombinant DTX3 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies on its enzymatic properties, substrate specificity, and interactions. Its applications span mechanistic research in oncogenesis, immune regulation, and neurodegenerative diseases, as well as drug discovery targeting ubiquitination pathways. Structural studies using recombinant DTX3 have further clarified its role in scaffolding protein assemblies. Current research focuses on its dual roles in tumor suppression/promotion and therapeutic potential in modulating immune responses.
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