| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNC1LI2 |
| Uniprot No | O43237 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTLMTKLQGAEHGKKGRGLEYLYLSVHDEDRDDHTRCNVWILDGDLYHKGLLKFAVSAESLPETLVIFVADMSRPWTVMESLQKWASVLREHIDKMKIPPEKMRELERKFVKDFQDYMEPEEGCQGSPQRRGPLTSGSDEENVALPLGDNVLTHNLGIPVLVVCTKCDAVSVLEKEHDYRDEHLDFIQSHLRRFCLQYGAALIYTSVKEEKNLDLLYKYIVHKTYGFHFTTPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVHDKELAAEDEQVFLMKQQSLLAKQPATPTRASESPARGPSGSPRTQGRGGPASVPSSSPGTSVKKPDPNIKNNAASEGVLASFFNSLLSKKTGSPGSPGAGGVQSTAKNSGQKTVLSNVQEELDRMTRKPDSMVTNSSTENEA |
| 分子量 | 80.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DYNC1LI2蛋白的3篇参考文献的简要列表，基于领域内常见研究方向整理：
1. **文献名称**：**"DYNC1LI2 interacts with LIS1 to regulate mitotic spindle orientation in neural development"**
**作者**：Smith A, et al.
**摘要**：该研究通过重组人DYNC1LI2蛋白与LIS1的体外结合实验，揭示两者在神经细胞分裂过程中调控纺锤体定向的分子机制，表明DYNC1LI2在神经发育障碍中可能发挥关键作用。
2. **文献名称**：**"Structural basis of the interaction between cytoplasmic dynein and NUDC through DYNC1LI2"**
**作者**：Chen L, et al.
**摘要**：研究人员利用重组表达的DYNC1LI2蛋白与NUDC共结晶，解析了二者复合体的三维结构，阐明了其如何介导动力蛋白与细胞核迁移相关蛋白的功能性结合。
3. **文献名称**：**"Recombinant human DYNC1LI2 facilitates adenovirus intracellular transport by bridging dynein and viral capsids"**
**作者**：Wang Y, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化重组人DYNC1LI2蛋白，证明其作为适配器蛋白直接连接腺病毒衣壳与动力蛋白复合体，促进病毒沿微管的逆向运输过程。
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**注**：以上文献信息为模拟示例，实际引用时需根据具体论文内容核对。若需真实文献，建议通过PubMed或Google Scholar检索关键词“DYNC1LI2 recombinant”“human DYNC1LI2 expression”获取最新研究。
DYNC1LI2 (Dynein Cytoplasmic 1 Light Intermediate Chain 2) is a key component of the cytoplasmic dynein-1 complex, a motor protein responsible for microtubule-mediated intracellular transport. This protein functions as a light intermediate chain, bridging the dynein heavy chain and cargo adaptors, thereby facilitating the movement of vesicles, organelles, and macromolecules toward the minus end of microtubules. It plays critical roles in neuronal trafficking, mitotic spindle positioning, and cell division.
Structurally, DYNC1LI2 contains conserved domains for binding dynein subunits (e.g., DYNC1H1) and regulatory partners like LIS1. which is associated with neurodevelopmental disorders. Dysregulation of DYNC1LI2 has been implicated in neurological diseases and cancers due to impaired cargo transport or mitotic errors. Recombinant human DYNC1LI2 is typically expressed in prokaryotic or eukaryotic systems for functional studies, enabling researchers to investigate its interactions, post-translational modifications, and mutational impacts. Its recombinant form also aids in structural analyses, drug screening, and modeling dynein-related pathologies, offering insights into therapeutic strategies targeting intracellular transport mechanisms.
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