| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYRK4 |
| Uniprot No | Q9NR20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MPASELKASEIPFHPSIKTQDPKAEEKSPKKQKVTLTAAEALKLFKNQLSPYEQSEILGYAELWFLGLEAKKLDTAPEKFSKTSFDDEHGFYLKVLHDHIAYRYEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWIHQSRNLKPQPRPQTLRKSNSFFPSETRKDKVQGCHHSSRKADEITKETTEKTKDSPTKHVQHSGDQQDCLQHGADTVQLPQLVDAPKKSEAAVGAEVSMTSPGQSKNFSLKNTNVLPPIV |
| 分子量 | 86 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DYRK4蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**：*Structural and Functional Analysis of Human DYRK4: A Protein Kinase Implicated in Cell Cycle Regulation*
**作者**：Smith J, et al.
**摘要**：该研究通过体外表达和纯化重组人DYRK4蛋白，解析了其晶体结构，并揭示其激酶活性依赖于Thr-173自磷酸化。实验表明，DYRK4通过磷酸化p53调控细胞周期停滞，可能参与肿瘤抑制过程。
2. **文献名称**：*DYRK4 Kinase Activity Modulates Neuronal Differentiation via MAPK Signaling*
**作者**：Chen L, Wang H.
**摘要**：本文报道了重组DYRK4蛋白在神经干细胞中的功能，发现DYRK4通过激活MAPK/ERK通路促进神经元分化。敲低DYRK4导致分化停滞，而重组蛋白的补充可挽救表型，提示其在神经发育中的关键作用。
3. **文献名称**：*Development of a High-Throughput Screening Assay for DYRK4 Inhibitors Using Recombinant Protein*
**作者**：Kimura T, et al.
**摘要**：研究团队利用重组DYRK4蛋白建立了一种基于荧光的激酶活性检测方法，筛选出多个小分子抑制剂，并验证其特异性。这些抑制剂为研究DYRK4在癌症和神经退行性疾病中的治疗潜力提供了工具。
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**注**：以上文献为示例，实际引用时需根据具体研究内容检索真实文献(如PubMed、Web of Science等数据库)。若需要查找真实文献，可尝试关键词：`Recombinant human DYRK4 + kinase activity/structure/function`。
**Background of Human Recombinant DYRK4 Protein**
DYRK4 (Dual-specificity tyrosine phosphorylation-regulated kinase 4) belongs to the DYRK family of serine/threonine and tyrosine kinases, which play critical roles in cell cycle regulation, differentiation, and survival. Structurally, DYRK4 contains a conserved kinase domain and distinct N-terminal regions that regulate its subcellular localization and activity. Unlike the well-studied DYRK1A and DYRK1B, DYRK4 is less characterized but is implicated in processes such as ciliogenesis, spermatogenesis, and possibly neurological functions.
The recombinant human DYRK4 protein is engineered using expression systems (e.g., *E. coli* or insect cells*) to produce a highly purified, catalytically active form for research. Its recombinant production enables detailed studies on substrate specificity, enzymatic kinetics, and interactions with regulatory partners. DYRK4 autophosphorylates its tyrosine residue during maturation, a hallmark of DYRKs, and phosphorylates downstream targets involved in cell cycle control and transcriptional regulation.
Research on DYRK4 has gained interest due to its potential links to diseases. Overexpression or dysregulation of DYRK family members is associated with cancer, neurodegenerative disorders (e.g., Alzheimer’s), and developmental defects. Inhibitors targeting DYRKs are under exploration for therapeutics, making recombinant DYRK4 valuable for drug screening and mechanistic studies. Despite progress, many aspects of DYRK4’s physiological roles and pathological contributions remain unclear, driving ongoing investigations into its signaling networks and tissue-specific functions.
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