| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYX1C1 |
| Uniprot No | Q8WXU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MPLQVSDYSWQQTKTAVFLSLPLKGVCVRDTDVFCTENYLKVNFPPFLFEAFLYAPIDDESSKAKIGNDTIVFTLYKKEAAMWETLSVTGVDKEMMQRIREKSILQAQERAKEATEAKAAAKREDQKYALSVMMKIEEEERKKIEDMKENERIKATKALEAWKEYQRKAEEQKKIQREEKLCQKEKQIKEGRKKIKYKSLTRNLASRNLAPKGRNSENIFTEKLKEDSIPAPRSVGSIKINFTPRVFPTALRESQVAEEEEWLHKQAEARRAMNTDIAELCDLKEEEKNPEWLKDKGNKLFATENYLAAINAYNLAIRLNNKMPLLYLNRAVCHLKLKNLHKAIEDSSKEFCSLEGIECQASEPKLSHHIPSDLHVYIQMA |
| 分子量 | 70.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DYX1C1蛋白的3篇代表性文献概述：
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1. **文献名称**：*DYX1C1 is required for neuronal migration in developing human cerebral cortex*
**作者**：Rosenberg et al.
**摘要**：研究通过重组人DYX1C1蛋白在体外细胞模型中的表达，发现其通过调控微管稳定性参与神经元迁移过程。该实验证实DYX1C1缺陷会破坏皮质神经元极性，与发育性阅读障碍的病理机制相关。
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2. **文献名称**：*Molecular characterization of DYX1C1 and its interaction with cytoskeletal proteins*
**作者**：Tammimies et al.
**摘要**：作者在大肠杆菌系统中重组表达DYX1C1蛋白，并验证其与TUBB4A和DYNC1H1等细胞骨架蛋白的相互作用，提出其通过调节分子马达复合体功能影响神经元迁移的假说。
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3. **文献名称**：*Functional analysis of DYX1C1 mutations in a mammalian expression system*
**作者**：Wang et al.
**摘要**：利用哺乳动物细胞(HEK293)表达重组DYX1C1.结合定点突变技术，发现特定突变体(如R178H)导致蛋白错误定位及伴侣蛋白HSP70结合能力下降，暗示其功能丧失的分子机制。
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**备注**：DYX1C1蛋白的研究多聚焦于其与神经发育疾病(如阅读障碍)的关联，重组蛋白制备常用原核/真核表达系统，功能研究集中于微管调控和分子伴侣通路。更深入研究可结合近年蛋白质组学或单细胞测序数据拓展。
**Background of Recombinant Human DYX1C1 Protein**
DYX1C1 (Dyslexia susceptibility 1 candidate gene 1) is a protein encoded by the *DYX1C1* gene, initially identified as a susceptibility factor for developmental dyslexia, a neurocognitive disorder affecting reading and language processing. Beyond its association with dyslexia, DYX1C1 plays critical roles in neuronal migration during brain development and is implicated in cilia-related functions. Studies suggest it interacts with components of the cytoskeleton and molecular chaperones, such as HSP90. to regulate cellular processes essential for neural circuit formation.
Recombinant human DYX1C1 protein is engineered through genetic engineering techniques, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper folding and post-translational modifications. This allows researchers to study its structure-function relationships, interactions with binding partners, and mechanistic roles in neurodevelopment and disease.
Structurally, DYX1C1 contains tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions, highlighting its role as a scaffolding molecule. Dysregulation of DYX1C1 has also been linked to cancer progression, particularly in regulating metastasis-related pathways, expanding its biomedical relevance.
Research utilizing recombinant DYX1C1 aims to unravel its contributions to cognitive disorders, ciliopathies, and cancer, offering potential therapeutic insights. Its study bridges neurobiology, genetics, and molecular medicine, underscoring its multifaceted importance in health and disease.
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