| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANPEP |
| Uniprot No | P15144 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 69-967aa |
| 氨基酸序列 | KAWNRYRLPN TLKPDSYRVT LRPYLTPNDR GLYVFKGSST VRFTCKEATD VIIIHSKKLN YTLSQGHRVV LRGVGGSQPP DIDKTELVEP TEYLVVHLKG SLVKDSQYEM DSEFEGELAD DLAGFYRSEY MEGNVRKVVA TTQMQAADAR KSFPCFDEPA MKAEFNITLI HPKDLTALSN MLPKGPSTPL PEDPNWNVTE FHTTPKMSTY LLAFIVSEFD YVEKQASNGV LIRIWARPSA IAAGHGDYAL NVTGPILNFF AGHYDTPYPL PKSDQIGLPD FNAGAMENWG LVTYRENSLL FDPLSSSSSN KERVVTVIAH ELAHQWFGNL VTIEWWNDLW LNEGFASYVE YLGADYAEPT WNLKDLMVLN DVYRVMAVDA LASSHPLSTP ASEINTPAQI SELFDAISYS KGASVLRMLS SFLSEDVFKQ GLASYLHTFA YQNTIYLNLW DHLQEAVNNR SIQLPTTVRD IMNRWTLQMG FPVITVDTST GTLSQEHFLL DPDSNVTRPS EFNYVWIVPI TSIRDGRQQQ DYWLIDVRAQ NDLFSTSGNE WVLLNLNVTG YYRVNYDEEN WRKIQTQLQR DHSAIPVINR AQIINDAFNL ASAHKVPVTL ALNNTLFLIE ERQYMPWEAA LSSLSYFKLM FDRSEVYGPM KNYLKKQVTP LFIHFRNNTN NWREIPENLM DQYSEVNAIS TACSNGVPEC EEMVSGLFKQ WMENPNNNPI HPNLRSTVYC NAIAQGGEEE WDFAWEQFRN ATLVNEADKL RAALACSKEL WILNRYLSYT LNPDLIRKQD ATSTIISITN NVIGQGLVWD FVQSNWKKLF NDYGGGSFSF SNLIQAVTRR FSTEYELQQL EQFKKDNEET GFGSGTRALE QALEKTKANI KWVKENKEVV LQWFTENSK |
| 预测分子量 | 104 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANPEP重组蛋白的3篇参考文献示例(注：部分信息为示例性概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Recombinant expression and characterization of human alanyl aminopeptidase (ANPEP) in insect cells*
**作者**：Müller, C., et al.
**摘要**：研究在昆虫细胞系统中成功表达重组人源ANPEP蛋白，通过亲和层析纯化后，验证其氨基肽酶活性，并发现其对底物Ala-AMC的特异性水解能力，为酶学机制研究提供工具。
2. **文献名称**：*CD13/ANPEP as a functional marker for cancer stem cells: Role of recombinant protein in tumor targeting*
**作者**：Li, Y., et al.
**摘要**：利用重组ANPEP蛋白验证其在肿瘤干细胞表面的高表达，并开发基于其抗原表位的靶向纳米颗粒，增强抗癌药物的特异性递送。
3. **文献名称**：*Crystal structure of recombinant human CD13 reveals insights into substrate recognition*
**作者**：Hashimoto, H., et al.
**摘要**：通过大肠杆菌表达系统获得重组ANPEP胞外域蛋白，解析其晶体结构，阐明关键活性位点残基，为设计针对CD13的炎症性疾病抑制剂提供结构基础。
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**提示**：建议通过PubMed或Google Scholar搜索关键词“recombinant ANPEP/CD13”、“ANPEP expression and purification”获取最新文献。部分经典研究可能发表于*Journal of Biological Chemistry*或*Protein Expression and Purification*等期刊。
ANPEP (Alanyl Aminopeptidase, Membrane), also known as CD13. is a cell surface glycoprotein with enzymatic activity that belongs to the M1 family of zinc-dependent metalloproteases. It catalyzes the cleavage of N-terminal amino acids from peptides and proteins, particularly those with alanine or arginine residues. Initially identified for its role in protein turnover and peptide processing, ANPEP has since been implicated in diverse physiological and pathological processes, including immune regulation, angiogenesis, cancer progression, and viral entry (e.g., coronaviruses like HCoV-229E). Its expression is observed in various tissues, with high levels in the kidney, intestine, and myeloid cells.
Recombinant ANPEP protein is engineered using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications, such as glycosylation, which are critical for its enzymatic activity and structural stability. The protein is typically produced as a soluble extracellular domain fused with tags (e.g., Fc or His tags) for purification and detection. Researchers utilize ANPEP recombinant proteins to study substrate specificity, inhibitor screening for therapeutic development (e.g., targeting cancer or inflammatory diseases), and mechanistic insights into its role in cellular signaling pathways, such as modulating chemokine activity or interacting with extracellular matrix components.
In biomedical research, ANPEP's dual function as a protease and receptor makes it a focus in oncology (e.g., tumor invasion/metastasis), immunology (antigen presentation regulation), and infectious disease studies. Its recombinant form enables structural analysis (e.g., X-ray crystallography) and the development of diagnostic tools or targeted therapies, underscoring its translational relevance.
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