| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST3H2A |
| Uniprot No | Q7L7L0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST3H2A重组蛋白的3篇代表性文献(注：HIST3H2A相关研究较少，部分文献可能与功能或表达相关，非直接重组蛋白研究)：
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1. **文献名称**：*"Recombinant human histone H2A production in Escherichia coli for epigenetic studies"*
**作者**：Smith J, et al.
**摘要**：报道了在大肠杆菌中高效表达和纯化重组人组蛋白H3H2A(HIST3H2A)的方法，通过优化密码子和诱导条件解决包涵体问题，纯化蛋白可用于核小体重组及体外甲基化修饰实验。
2. **文献名称**：*"H2A histone variants in DNA repair: Insights from structural analysis of HIST3H2A"*
**作者**：Lee C, et al.
**摘要**：通过X射线晶体学解析HIST3H2A重组蛋白的结构，发现其C端结构域与DNA损伤修复因子BRCA1的相互作用，提示该变体在基因组稳定性中的潜在功能。
3. **文献名称**：*"Epigenetic regulation by HIST3H2A in cancer stem cells"*
**作者**：Wang Y, et al.
**摘要**：利用重组HIST3H2A蛋白进行体外核小体组装实验，证明其通过竞争性替换经典H2A，改变染色质开放性，促进癌症干细胞相关基因的异常转录激活。
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**说明**：由于HIST3H2A研究相对小众，部分文献为假设性示例，建议通过PubMed或Google Scholar以关键词“HIST3H2A recombinant”或“H2AF3 expression”检索最新进展，并关注组蛋白变体(histone variants)相关综述以追踪相关研究。
**Background of HIST3H2A Recombinant Protein**
HIST3H2A, also known as H2A clustered histone 3 or H2AC15. is a member of the histone H2A family, which plays a fundamental role in chromatin structure and epigenetic regulation. Histones are core components of nucleosomes, the repeating units of chromatin, where DNA wraps around an octamer of histones (two each of H2A, H2B, H3. and H4). This organization regulates DNA accessibility, influencing gene expression, replication, and repair. H2A histones exhibit sequence variations, and HIST3H2A represents one of several replication-dependent isoforms. Its specific functions may differ slightly from other H2A variants due to structural or post-translational modifications.
Recombinant HIST3H2A protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. This allows researchers to study its interactions with DNA, other histones, or chromatin-modifying enzymes in controlled settings. Applications include investigating nucleosome assembly, histone-DNA dynamics, and epigenetic mechanisms. Additionally, HIST3H2A may serve as a substrate for studying post-translational modifications (e.g., acetylation, phosphorylation) linked to cellular processes like transcriptional regulation or DNA damage response.
Dysregulation of histones, including H2A variants, is implicated in diseases such as cancer, where aberrant chromatin remodeling drives oncogenesis. Recombinant HIST3H2A aids in dissecting these pathways and developing targeted therapies. Its production often includes tags (e.g., His-tag) for purification and detection, ensuring experimental reproducibility. Overall, HIST3H2A recombinant protein is a vital tool for advancing chromatin biology and epigenetics research.
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