| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EYA1 |
| Uniprot No | Q99502 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-592aa |
| 氨基酸序列 | MEMQDLTSPH SRLSGSSESP SGPKLGNSHI NSNSMTPNGT EVKTEPMSSS ETASTTADGS LNNFSGSAIG SSSFSPRPTH QFSPPQIYPS NRPYPHILPT PSSQTMAAYG QTQFTTGMQQ ATAYATYPQP GQPYGISSYG ALWAGIKTEG GLSQSQSPGQ TGFLSYGTSF STPQPGQAPY SYQMQGSSFT TSSGIYTGNN SLTNSSGFNS SQQDYPSYPS FGQGQYAQYY NSSPYPAHYM TSSNTSPTTP STNATYQLQE PPSGITSQAV TDPTAEYSTI HSPSTPIKDS DSDRLRRGSD GKSRGRGRRN NNPSPPPDSD LERVFIWDLD ETIIVFHSLL TGSYANRYGR DPPTSVSLGL RMEEMIFNLA DTHLFFNDLE ECDQVHIDDV SSDDNGQDLS TYNFGTDGFP AAATSANLCL ATGVRGGVDW MRKLAFRYRR VKEIYNTYKN NVGGLLGPAK REAWLQLRAE IEALTDSWLT LALKALSLIH SRTNCVNILV TTTQLIPALA KVLLYGLGIV FPIENIYSAT KIGKESCFER IIQRFGRKVV YVVIGDGVEE EQGAKKHAMP FWRISSHSDL MALHHALELE YL |
| 分子量 | 64.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EYA1蛋白的3篇参考文献示例(内容为虚构，仅用于格式演示)：
1. **文献名称**: *Structural and functional analysis of recombinant human EYA1 tyrosine phosphatase*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统重组表达了人EYA1蛋白的催化结构域，解析了其晶体结构，并验证了其酪氨酸磷酸酶活性。结果表明，EYA1通过结合SIX1蛋白调控下游基因的发育相关信号通路。
2. **文献名称**: *EYA1 mutations disrupt interaction with SIX1 and cause branchio-oto-renal syndrome*
**作者**: Miller KE, et al.
**摘要**: 研究利用重组野生型和突变型EYA1蛋白，证实了特定致病突变(如R561H)会破坏EYA1与SIX1的互作能力，导致胚胎期耳肾发育异常，解释了Branchio-oto-renal综合征的分子机制。
3. **文献名称**: *Recombinant EYA1 promotes DNA repair via dephosphorylation of γ-H2AX in cancer cells*
**作者**: Chen Y, et al.
**摘要**: 通过哺乳动物细胞表达体系获得重组人EYA1.发现其磷酸酶活性可通过去磷酸化组蛋白H2AX的Ser139位点，调控DNA损伤修复，提示EYA1在癌症治疗耐药性中的潜在作用。
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*注：以上文献及摘要为示例性内容，实际研究中请以真实发表的论文为准。*
Recombinant human EYA1 protein is a biologically engineered version of the Eyes Absent Homolog 1 (EYA1) protein, which plays critical roles in embryonic development and cellular regulation. EYA1 belongs to the EYA family of proteins, initially identified in *Drosophila* as essential for eye development. In humans, EYA1 functions as both a transcriptional coactivator and a tyrosine phosphatase, participating in organogenesis, particularly in the formation of the kidneys, inner ear, and branchial arches. It interacts with SIX transcription factors to regulate gene expression during tissue development and repair.
Mutations in the *EYA1* gene are linked to congenital disorders such as Branchio-Oto-Renal (BOR) syndrome, characterized by hearing loss, renal anomalies, and craniofacial defects. Recombinant EYA1 is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies of its dual enzymatic and scaffolding functions. Researchers use it to investigate signaling pathways (e.g., DNA damage response, Hippo pathway), disease mechanisms, and potential therapeutic targets. Its phosphatase activity, mediated by the C-terminal domain, is of particular interest in cancer research due to its role in cell proliferation and apoptosis. By providing a purified, functional form of EYA1. recombinant protein technology accelerates structural and functional analyses, aiding drug discovery and understanding developmental biology.
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