| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EYA3 |
| Uniprot No | Q99504 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MEEEQDLPEQPVKKAKMQESGEQTISQVSNPDVSDQKPETSSLASNLPMSEEIMTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFASSTNASLISTSSTIANIPAAAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSSGDPSTSPSLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDSELERVFLWDLDETIIIFHSLLTGSYAQKYGKDPTVVIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGSGGSGSHGSSVGVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSLLLIQSRKNCVNVLITTTQLVPALAKVLLYGLGEIFPIENIYSATKIGKESCFERIVSRFGKKVTYVVIGDGRDEEIAAKQQLYFLDMEALGCQLEPTALILFIQLSGNLSNYNKL |
| 分子量 | 59 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EYA3蛋白的3篇参考文献示例(注：由于无法实时访问文献数据库，以下内容为基于领域知识的模拟示例，实际文献需通过学术平台检索)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human EYA3 Phosphatase in Cancer Cell Lines"*
**作者**: Smith, J. et al.
**摘要**: 该研究通过大肠杆菌表达系统成功制备了重组人EYA3蛋白，并验证其磷酸酶活性。作者发现EYA3通过去磷酸化Hippo通路关键蛋白促进癌细胞迁移，提示其在肿瘤发生中的潜在作用。
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2. **文献名称**: *"Structural Insights into the DNA Repair Function of EYA3: A Recombinant Protein Study"*
**作者**: Lee, H. & Zhang, Y.
**摘要**: 利用昆虫细胞表达系统纯化出高活性的重组人EYA3蛋白，通过X射线晶体学解析其三维结构。研究发现EYA3与DNA损伤修复蛋白相互作用，其磷酸酶结构域对修复双链断裂至关重要。
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3. **文献名称**: *"Role of Recombinant EYA3 in TGF-β Signaling Pathway Regulation"*
**作者**: Wang, R. et al.
**摘要**: 研究通过哺乳动物细胞表达重组EYA3.发现其通过结合Smad蛋白增强TGF-β信号传导，并证明其磷酸酶活性对成纤维细胞向肌成纤维细胞转化具有调控作用，为纤维化疾病提供新靶点。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词：**"recombinant human EYA3" "phosphatase" "cancer" "DNA repair"**。
The EYA3 (Eyes Absent Homolog 3) protein is a member of the EYA family of tyrosine phosphatases and transcriptional coactivators, playing dual enzymatic and scaffolding roles in cellular processes. EYA3 contains an N-terminal transactivation domain and a C-terminal phosphatase domain, enabling its involvement in DNA repair, apoptosis, and developmental pathways. It interacts with HTH (Hip, Tshz3. and Six1) transcription factors to regulate organogenesis, notably in kidney, muscle, and inner ear development. Dysregulation of EYA3 has been linked to cancer progression, cardiovascular diseases, and immune responses, with overexpression observed in breast, lung, and colorectal cancers, where it promotes metastasis through TGF-β and EGFR signaling. Recombinant human EYA3 protein is typically produced in prokaryotic (E. coli) or eukaryotic (insect/mammalian) expression systems, often fused with tags (e.g., His-tag) for purification. Its phosphatase activity is magnesium-dependent, distinguishing it from classical tyrosine phosphatases. Studies utilize recombinant EYA3 to investigate its structural dynamics, substrate specificity (e.g., phosphorylated Tau in neurodegeneration), and therapeutic targeting. Recent research explores its role in tumor immunomodulation and as a potential biomarker for early cancer detection or therapeutic intervention in tyrosine kinase inhibitor-resistant malignancies.
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