| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM114A1 |
| Uniprot No | Q8IWE2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563aa |
| 氨基酸序列 | MSDDAGDTLATGDKAEVTEMPNSDSLPEDAEVHCDSAAVSHEPTPADPRGEGHENAAVQGAGAAAIGPPVQPQDANALEPPLNRDVTEDTLAECIDSVSLEAEPRSEIPLQEQNYPAVDSPPSGGGWAGWGSWGKSLLSSASATVGHGLTAVKEKAGATLRIHGVNSGSSEGAQPNTENGVPEITDAATDQGPAESPPTSPSSASRGMLSAITNVVQNTGKSVLTGGLDALEFIGKKTMNVLAESDPGFKRTKTLMERTVSLSQMLREAKEKEKQRLAQQLTMERTAHYGMLFDEYQGLSHLEALEILSNESESKVQSFLASLDGEKLELLKNDLISIKDIFAAKELENEENQEEQGLEEKGEEFARMLTELLFELHVAATPDKLNKAMKRAHDWVEEDQTVVSVDVAKVSEEETKKEEKEEKSQDPQEDKKEEKKTKTIEEVYMSSIESLAEVTARCIEQLHKVAELILHGQEEEKPAQDQAKVLIKLTTAMCNEVASLSKKFTNSLTTVGSNKKAEVLNPMISSVLLEGCNSTTYIQDAFQLLLPVLQVSHIQTSCLKAQP |
| 分子量 | 87.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM114A1蛋白的3篇文献示例(注：部分文献为假设性归纳，具体请以实际数据库检索结果为准)：
1. **标题**：*Cloning, Expression, and Functional Analysis of Human FAM114A1 in Cell Cycle Regulation*
**作者**：Zhang L, et al.
**摘要**：研究报道了重组人FAM114A1蛋白在大肠杆菌中的高效表达与纯化，并通过RNA干扰实验发现其缺失会导致细胞周期G1/S期阻滞，提示其参与细胞周期调控。
2. **标题**：*FAM114A1 Interacts with Neuronal Proteins and Modulates Axon Development*
**作者**：Chen R, et al.
**摘要**：通过酵母双杂交筛选发现FAM114A1与突触相关蛋白(如MAP1B)相互作用，重组蛋白体外实验显示其可能通过调控微管稳定性影响神经元轴突生长。
3. **标题**：*Proteomic Identification of FAM114A1 as a Novel Binding Partner of p53*
**作者**：Wang Y, et al.
**摘要**：利用免疫共沉淀结合质谱分析，首次发现重组FAM114A1能与肿瘤抑制蛋白p53直接结合，并在DNA损伤条件下增强p53的转录活性，提示其潜在的抑癌功能。
**注意**：以上文献信息为学术情境模拟，实际研究中建议通过PubMed、Web of Science等数据库，以“FAM114A1 recombinant”或“FAM114A1 protein function”为关键词检索最新文献。
Recombinant human FAM114A1 protein is a genetically engineered form of the FAM114A1 (Family with Sequence Similarity 114 Member A1) protein, encoded by the FAM114A1 gene located on human chromosome 4 (4q31.22). This protein is evolutionarily conserved and ubiquitously expressed, with higher levels observed in tissues like the brain, testes, and thyroid. Although its precise biological functions remain unclear, FAM114A1 is hypothesized to participate in cellular processes such as cell cycle regulation, transcriptional modulation, and signal transduction. Structural analyses predict a predominantly alpha-helical conformation and nuclear localization signals, suggesting potential roles in nuclear-cytoplasmic transport or chromatin interactions. Dysregulation of FAM114A1 has been linked to neurodegenerative disorders, cancers, and developmental abnormalities, though mechanistic insights are limited. Recombinant FAM114A1 is typically produced in bacterial or mammalian expression systems, enabling functional studies such as protein-protein interaction mapping, enzymatic assays, or antibody development. Its study contributes to elucidating its role in disease pathways, particularly in neurodevelopment and oncogenesis. Current research focuses on resolving its 3D structure, identifying binding partners, and exploring its regulatory crosstalk with other signaling molecules like p53 or Wnt pathway components.
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