| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM160B2 |
| Uniprot No | Q86V87 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743aa |
| 氨基酸序列 | MLSRLGALLQ EAVGAREPSI DLLQAFVEHW KGITHYYIES TDESTPAKKT DIPWRLKQML DILVYEEQQQ AAAGEAGPCL EYLLQHKILE TLCTLGKAEY PPGMRQQVFQ FFSKVLAQVQ HPLLHYLSVH RPVQKLLRLG GTASGSVTEK EEVQFTTVLC SKIQQDPELL AYILEGKKIV GRKKACGEPT ALPKDTTSHG DKDCSHDGAP ARPQLDGESC GAQALNSHMP AETEELDGGT TESNLITSLL GLCQSKKSRV ALKAQENLLL LVSMASPAAA TYLVQSSACC PAIVRHLCQL YRSMPVFLDP ADIATLEGIS WRLPSAPSDE ASFPGKEALA AFLGWFDYCD HLITEAHTVV ADALAKAVAE NFFVETLQPQ LLHVSEQSIL TSTALLTAML RQLRSPALLR EAVAFLLGTD RQPEAPGDNP HTLYAHLIGH CDHLSDEISI TTLRLFEELL QKPHEGIIHS LVLRNLEGRP YVAWGSPEPE SYEDTLDLEE DPYFTDSFLD SGFQTPAKPR LAPATSYDGK TAVTEIVNSF LCLVPEEAKT SAFLEETGYD TYVHDAYGLF QECSSRVASW GWPLTPTPLD PHEPERPFFE GHFLRVLFDR MSRILDQPYS LNLQVTSVLS RLALFPHPHI HEYLLDPYIS LAPGCRSLFS VLVRVIGDLM QRIQRVPQFP GKLLLVRKQL TGQAPGEQLD HQTLLQGVVV LEEFCKELAA IAFVKFPPHD PRQNVSPAPE GQV |
| 分子量 | 82.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM160B2蛋白的模拟参考文献示例，实际文献需通过学术数据库验证：
1. **"Recombinant expression and functional characterization of FAM160B2 in microtubule stabilization"**
*作者：Zhang Y, et al.*
摘要：本研究利用大肠杆菌系统成功表达并纯化重组人FAM160B2蛋白，发现其通过结合微管蛋白增强微管稳定性，揭示了其在细胞骨架动力学中的作用。
2. **"FAM160B2 regulates Golgi structure by interacting with the COPI complex"**
*作者：Lee S, et al.*
摘要：通过重组FAM160B2蛋白的体外实验，证明其与COPI囊泡复合物结合，调控高尔基体膜的结构完整性及细胞内运输功能。
3. **"FAM160B2 as a novel binding partner of Rab GTPases in endosomal trafficking"**
*作者：Smith J, et al.*
摘要：利用重组FAM160B2蛋白进行Pull-down实验，发现其与Rab5和Rab7相互作用，影响内体运输和溶酶体降解途径。
4. **"Structural insights into the kinesin-binding domain of FAM160B2 via X-ray crystallography"**
*作者：Johnson R, et al.*
摘要：通过重组蛋白结晶技术解析FAM160B2的三维结构，鉴定出一个新型驱动蛋白(kinesin)结合域，为研究其运输功能提供分子基础。
**注意**：上述内容为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索最新文献。若FAM160B2相关研究有限，建议扩展至其家族成员(如FAM160B1)或功能相关关键词(如微管结合蛋白、细胞内运输)。
FAM160B2 (Family with sequence similarity 160 member B2) is a microtubule-associated protein involved in intracellular transport and cytoskeletal organization. It is recognized as a paralog of FAM160A1 and belongs to a conserved protein family characterized by coiled-coil domains. Structurally, FAM160B2 contains a kinesin light chain-like domain, suggesting functional interactions with motor proteins such as kinesins. Studies indicate its role in regulating kinesin-mediated cargo transport along microtubules, particularly in maintaining Golgi apparatus structure and facilitating vesicle trafficking.
The protein is ubiquitously expressed in human tissues, with higher levels observed in the brain, testes, and kidneys. Its interaction with KIF1C (kinesin family member 1C) highlights its importance in cell migration and adhesion processes. Dysregulation of FAM160B2 has been implicated in various pathologies, including cancer progression and neurodegenerative disorders. For instance, altered expression patterns are associated with colorectal cancer metastasis and Parkinson's disease pathogenesis.
Recombinant human FAM160B2 protein is typically produced in E. coli or mammalian expression systems for biochemical studies. It serves as a critical tool for investigating microtubule dynamics, cellular transport mechanisms, and disease-related molecular pathways. Current research focuses on characterizing its post-translational modifications and exploring its potential as a therapeutic target or diagnostic biomarker.
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