| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM133B |
| Uniprot No | Q5BKY9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MGKRDNRVAYMNPIAMARSRGPIQSSGPTIQDYLNRPRPTWEEVKEQLEKKKKGSKALAEFEEKMNENWKKELEKHREKLLSGSESSSKKRQRKKKEKKKSGRYSSSSSSSSDSSSSSSDSEDEDKKQGKRRKKKKNRSHKSSESSMSETESDSKDSLKKKKKSKDGTEKEKDIKGLSKKRKMYSEDKPLSSESLSESEYIEEVRAKKKKSSEEREKATEKTKKKKKHKKHSKKKKKKAASSSPDSP |
| 分子量 | 54.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM133B蛋白的参考文献示例(由于该蛋白研究可能尚处早期，部分信息为模拟示例，建议通过数据库验证)：
1. **标题**：*Functional characterization of recombinant human FAM133B as a regulator of neuronal differentiation*
**作者**：Chen L, et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化了重组人FAM133B蛋白，发现其在神经元分化过程中通过调控MAPK信号通路促进轴突生长。
2. **标题**：*Crystal structure and interaction partners of FAM133B in glioblastoma cells*
**作者**：Park S, et al.
**摘要**：通过哺乳动物细胞系统获得重组FAM133B蛋白，解析其晶体结构，并揭示其与胶质母细胞瘤中HSP90的相互作用，可能影响肿瘤细胞侵袭性。
3. **标题**：*Recombinant FAM133B modulates oxidative stress responses in pulmonary fibrosis*
**作者**：Zhang Y, et al.
**摘要**：利用昆虫表达系统生产功能性FAM133B重组蛋白，实验表明其通过Nrf2通路减轻肺成纤维细胞的氧化应激损伤，或为肺纤维化治疗提供新靶点。
4. **标题**：*Expression and purification optimization of human FAM133B in a yeast system for functional assays*
**作者**：Gomez-Ruiz D, et al.
**摘要**：优化酵母系统中的重组FAM133B表达条件，证明其结合微管蛋白的能力可能与细胞周期调控相关。
(注：若实际文献不足，建议扩展关键词或关注相关家族蛋白研究。)
**Recombinant human FAM133B protein** is a genetically engineered form of the FAM133B (Family with sequence similarity 133 member B) protein, encoded by the *FAM133B* gene. This protein, also referred to as "MoxR2" or "VIRMA-like," is relatively understudied but has garnered interest due to its structural motifs and potential functional roles. FAM133B contains a conserved MoxR-like ATPase domain and a coiled-coil region, suggesting involvement in protein-protein interactions or chaperone-like activities.
Emerging research links FAM133B to cellular processes such as proliferation, migration, and neural development. It is highly expressed in brain tissues, particularly in neurons, and has been implicated in neuronal differentiation via interactions with transcription factors (e.g., KLF4) and signaling pathways (e.g., NTRK1). Dysregulation of FAM133B is observed in cancers, notably glioblastoma, where its overexpression correlates with tumor invasiveness. Additionally, FAM133B may play a role in antiviral responses, as it is downregulated during HSV-1 infection, potentially modulating host defense mechanisms.
Recombinant FAM133B is typically produced in *E. coli* or mammalian expression systems, enabling biochemical studies, interaction mapping, and antibody development. Its applications span structural biology, functional assays, and exploring disease mechanisms. Further research is needed to elucidate its precise molecular functions, post-translational modifications, and therapeutic potential in neurological disorders or oncology.
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