Recombinant Human FAM12A Protein

Recombinant human FAM12A protein, also known as CXorf67 or FAM12A, is a product of the FAM12A gene located on the X chromosome. This protein belongs to the FAM12 family, which shares conserved sequences but lacks clear structural homology to other known protein families. FAM12A is ubiquitously expressed in tissues, with higher levels observed in organs like the liver and ovaries, suggesting roles in cellular homeostasis. Its precise molecular function remains under investigation, but studies link it to cell proliferation, differentiation, and tumor suppression.

Recombinant FAM12A is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems (e.g., mammalian cell lines) to ensure proper folding and post-translational modifications. Purified versions often include tags (e.g., His-tag) for affinity chromatography. Structural analyses predict FAM12A contains α/β-hydrolase folds, hinting at potential enzymatic or scaffold activities.

Emerging evidence implicates FAM12A in cancer biology. Downregulation or mutations in FAM12A correlate with poor prognosis in malignancies such as ovarian and hepatocellular carcinomas, likely through dysregulation of cell cycle checkpoints or apoptosis pathways. Additionally, it may interact with signaling molecules like TP53 or AKT, though mechanistic details remain unclear. Beyond oncology, FAM12A has potential roles in metabolic and developmental disorders.

Research applications include in vitro functional studies, antibody development, and exploration of therapeutic targeting strategies. Its recombinant form enables biochemical characterization and validation of clinical associations. Further studies are needed to decode its molecular interactions and pathophysiological significance.