关键信息
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基因名
PARD6B
- 应用
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别名
PARD6B; PAR6BPartitioning defective 6 homolog beta; PAR-6 beta; PAR-6B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BYG5
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表达区间
1-372 aa
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氨基酸序列
MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD YSAFGTDTLI KKKNVLTNVL RPDNHRKKPH IVISMPQDFR PVSSIIDVDI LPETHRRVRL YKYGTEKPLG FYIRDGSSVR VTPHGLEKVP GIFISRLVPG GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR NLIITVRPAN QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIED NGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN TEFETHAPDQ KLLEEDGTII TL
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分子量
41.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PARD6B, a member of the Par (partitioning defective) protein family, plays a crucial role in establishing cell polarity, which is vital for various cellular processes including tissue organization, cell differentiation, and signaling pathways. The protein is involved in the formation of tight junctions and is essential for the asymmetric distribution of cellular components during division. Recent studies have implicated PARD6B in various pathophysiological conditions, including cancer metastasis and developmental disorders. The recombinant expression of PARD6B protein allows researchers to explore its functional capabilities and interactions within the cell, facilitating a deeper understanding of its role in maintaining cellular architecture and dynamics. Utilizing advanced molecular biology techniques, scientists aim to elucidate the protein's structural characteristics and its involvement in signal transduction pathways. Additionally, generating PARD6B recombinant protein can serve as a valuable tool for developing targeted therapies that address the dysregulation of cell polarity in diseases. Overall, understanding PARD6B's function and mechanisms offers significant potential for advancing therapeutic strategies in related medical fields.
