| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM126B |
| Uniprot No | Q8IXS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MLGTDRCVVEEWLSEFKALPDTQITSYAATLHRKKTLVPALYKVIQDSNNELLEPVCHQLFELYRSSEVRLKRFTLQFLPELMWVYLRLTVSRDRQSNGCIEALLLGIYNLEIADKDGNNKVLSFTIPSLSKPSIYHEPSTIGSMALTEGALCQHDLIRVVYSDLHPQRETFTAQNRFEVLSFLMLCYNSAIVYMPASSYQSLCRMGSRVCVSGFPRQHEKHWKELCGRIVLDPEFMVQLLTGVYYAMYNGQWDLGQEVLDDIIYRAQLELFSQPLLVANAMKNSLPFDAPDSTQEGQKVLKVEVTPTVPRISRTAITTASIRRHRWRREGAEGVNGGEESVNLNDADEGFSSGASLSSQPIGTKPSSSSQRGSLRKVATGRSAKDKETASAIKSSESPRDSVVRKQYVQQPTDLSVDSVELTPMKKHLSLPAGQVVPKINSLSLIRTASASSSKSFDYVNGSQASTSIGVGTEGGTNLAANNANRYSTVSLQEDRLGQAGEGKELLSPGAPLTKQSRSPSFNMQLISQV |
| 分子量 | 85 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM126B蛋白的假设参考文献示例，简要概括其内容：
1. **Smith et al. (2020)**
*标题*：重组人FAM126B蛋白在大肠杆菌中的高效表达与纯化
*摘要*：该研究报道了通过大肠杆菌系统成功表达并纯化重组人FAM126B蛋白，揭示了其热稳定性和二聚体结构，为后续功能研究奠定基础。
2. **Zhang et al. (2018)**
*标题*：人FAM126B的晶体结构及其与HYCCIN复合物的互作分析
*摘要*：通过X射线晶体学解析了FAM126B的三维结构，发现其通过疏水界面与HYCCIN结合，提示二者在调控细胞膜相关信号通路中的协同作用。
3. **Lee and Kim (2021)**
*标题*：FAM126B在胶质母细胞瘤中的促癌机制研究
*摘要*：体外实验表明，FAM126B过表达显著增强胶质母细胞瘤细胞增殖和迁移，可能通过PI3K/AKT通路调控肿瘤进展。
4. **Johnson et al. (2019)**
*标题*：FAM126B突变导致腓骨肌萎缩症的分子机制
*摘要*：发现FAM126B的R183C突变破坏其与脂质结合能力，导致神经元髓鞘形成异常，重组野生型蛋白可部分恢复细胞模型中的功能缺陷。
*注：以上为假设性示例，实际文献需通过学术数据库检索确认。*
Recombinant human FAM126B protein is a biologically engineered form of the FAM126B (family with sequence similarity 126 member B) protein, also known as Hyccin. This protein is encoded by the *FAM126B* gene in humans and is primarily expressed in the central nervous system, particularly in oligodendrocytes and Schwann cells. FAM126B plays a critical role in cellular membrane organization and myelination processes. It interacts with phosphatidylinositol 4-kinase type IIIα (PI4KIIIα) to regulate phosphatidylinositol 4-phosphate (PI4P) synthesis, essential for maintaining plasma membrane integrity and vesicular trafficking.
Mutations in *FAM126B* are linked to hypomyelinating leukodystrophy-5 (HLD5), a rare autosomal recessive disorder characterized by defective myelin formation in the brain. This association highlights its importance in nervous system development. Recombinant FAM126B is typically produced in *E. coli* or mammalian expression systems, enabling studies on its structure-function relationships, molecular interactions, and disease mechanisms. It serves as a vital tool for elucidating myelin-related pathologies and screening therapeutic agents targeting demyelinating diseases. Purified recombinant protein often includes tags (e.g., His-tag) for detection and purification, ensuring utility in biochemical assays, antibody development, and cellular models.
×
