| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM122A |
| Uniprot No | Q96E09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-287aa |
| 氨基酸序列 | MAQEKMELDLELPPGTGGSPAEGGGSGGGGGLRRSNSAPLIHGLSDTSPVFQAEAPSARRNSTTFPSRHGLLLPASPVRMHSSRLHQIKQEEGMDLINRETVHEREVQTAMQISHSWEESFSLSDNDVEKSASPKRIDFIPVSPAPSPTRGIGKQCFSPSLQSFVSSNGLPPSPIPSPTTRFTTRRSQSPINCIRPSVLGPLKRKCEMETEYQPKRFFQGITNMLSSDVAQLSDPGVCVSSDTLDGNSSSAGSSCNSPAKVSTTTDSPVSPAQAASPFIPLDELSSK |
| 分子量 | 56.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM122A蛋白的3篇虚构参考文献范例(注：实际文献可能需要通过学术数据库查询)：
1. **文献名称**: "FAM122A as a novel regulator of PP2A phosphatase activity in cell cycle control"
**作者**: Chen L., et al.
**摘要**: 研究报道了FAM122A蛋白通过与PP2A磷酸酶复合物相互作用，抑制其活性，从而调控细胞周期G1/S期转换的分子机制，重组人FAM122A在体外实验中验证了其结合能力。
2. **文献名称**: "Structural insights into the tumor-suppressive role of FAM122A via X-ray crystallography"
**作者**: Zhang Y., et al.
**摘要**: 首次解析了重组人FAM122A蛋白的晶体结构，揭示其N端结构域包含关键的α-螺旋与β-折叠模体，可能参与调控与癌症相关信号通路(如Wnt通路)蛋白的互作。
3. **文献名称**: "FAM122A deficiency promotes hepatocellular carcinoma progression through stabilizing c-Myc"
**作者**: Wang Q., et al.
**摘要**: 利用重组FAM122A蛋白进行功能挽救实验，发现其通过泛素化途径降解c-Myc蛋白，抑制肝癌细胞增殖，提示FAM122A具有肿瘤抑制潜力。
注：上述内容为基于FAM122A已知生物学功能的模拟摘要，实际文献需查询PubMed或Google Scholar等平台。已知真实研究显示FAM122A与PP2A/CIP2A通路调控相关(PMID: 30686569)。
Recombinant human FAM122A protein is a biologically engineered form of the FAM122A (Family with Sequence Similarity 122A) protein, which plays regulatory roles in cellular processes such as cell cycle progression, apoptosis, and stress responses. FAM122A, also identified as a conserved intrinsic disordered protein, lacks well-defined structural domains, contributing to its dynamic interactions with molecular partners. Studies suggest it acts as a negative regulator of protein phosphatase 2A (PP2A), a tumor suppressor complex, by binding to its catalytic subunit and inhibiting phosphatase activity. This interaction may promote cell proliferation and survival, linking FAM122A to oncogenic pathways. Additionally, FAM122A is implicated in modulating p53 signaling under DNA damage, highlighting its dual role in stress response and cancer biology.
The recombinant protein is typically produced in Escherichia coli or mammalian expression systems, purified via affinity tags, and validated for functional assays. Its applications span in vitro studies exploring PP2A regulation, mechanisms of cell cycle checkpoints, and therapeutic targeting in cancers where FAM122A is overexpressed (e.g., liver or colorectal cancers). Despite progress, FAM122A’s full interactome and disease relevance remain under investigation, emphasizing the importance of recombinant tools in elucidating its pathophysiological roles.
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