| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM120B |
| Uniprot No | Q96EK7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-910aa |
| 氨基酸序列 | MGVRGLQGFVGSTCPHICTVVNFKELAEHHRSKYPGCTPTIVVDAMCCLRYWYTPESWICGGQWREYFSALRDFVKTFTAAGIKLIFFFDGMVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMFFIPSGLAVFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCPYFSISELCLESLDTVMLCREKLCESLGLCVADLPLLACLLGNDIIPEGMFESFRYKCLSSYTSVKENFDKKGNIILAVSDHISKVLYLYQGEKKLEEILPLGPNKALFYKGMASYLLPGQKSPWFFQKPKGVITLDKQVISTSSDAESREEVPMCSDAESRQEVPMCTGPESRREVPVYTDSEPRQEVPMCSDPEPRQEVPTCTGPESRREVPMCSDPEPRQEVPMCTGPEARQEVPMYTDSEPRQEVPMYTDSEPRQEVPMYTGSEPRQEVPMYTGPESRQEVPMYTGPESRQEVLIRTDPESRQEIMCTGHESKQEVPICTDPISKQEDSMCTHAEINQKLPVATDFEFKLEALMCTNPEIKQEDPTNVGPEVKQQVTMVSDTEILKVARTHHVQAESYLVYNIMSSGEIECSNTLEDELDQALPSQAFIYRPIRQRVYSLLLEDCQDVTSTCLAVKEWFVYPGNPLRHPDLVRPLQMTIPGGTPSLKILWLNQEPEIQVRRLDTLLACFNLSSSREELQAVESPFQALCCLLIYLFVQVDTLCLEDLHAFIAQALCLQGKSTSQLVNLQPDYINPRAVQLGSLLVRGLTTLVLVNSACGFPWKTSDFMPWNVFDGKLFHQKYLQSEKGYAVEVLLEQNRSRLTKFHNLKAVVCKACMKENRRITGRAHWGSHHAGRWGRQGSSYHRTGSGYSRSSQGQPWRDQGPGSRQYEHDQWRRY |
| 分子量 | 130.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM120B蛋白的3篇文献概览(注：文献为虚拟示例，仅供参考)：
1. **文献名称**：*FAM120B regulates oxidative stress response via interaction with Keap1-Nrf2 pathway*
**作者**：Li X. et al. (2022)
**摘要**：研究揭示了FAM120B通过重组蛋白体外结合实验，证实其与Keap1蛋白互作，调控Nrf2抗氧化信号通路，影响细胞在氧化应激中的存活能力。
2. **文献名称**：*Recombinant human FAM120B promotes adipogenesis via PPARγ activation*
**作者**：Zhang Y. et al. (2021)
**摘要**：通过表达并纯化重组人FAM120B蛋白，发现其能结合PPARγ转录因子，增强脂肪细胞分化，提示其在代谢综合征中的潜在调控作用。
3. **文献名称**：*Structural and functional analysis of FAM120B in RNA splicing machinery*
**作者**：Wang R. et al. (2020)
**摘要**：利用重组FAM120B蛋白进行晶体结构解析，发现其C端结构域参与结合U1 snRNP复合体，影响前体mRNA剪接，为相关肿瘤异常剪接机制提供依据。
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**备注**：以上文献信息为模拟生成，实际研究中建议通过数据库(如PubMed)以“FAM120B”或“recombinant FAM120B”为关键词查询最新进展。
Recombinant human FAM120B (family with sequence similarity 120B) is a protein encoded by the FAM120B gene, belonging to the SURF1 family. It functions as an oxidative stress-responsive protein and is implicated in cellular processes such as RNA metabolism, transcriptional regulation, and stress signaling. Structurally, FAM120B contains conserved nuclear localization signals and two coiled-coil domains, suggesting roles in protein-protein interactions and subcellular localization. It acts as a molecular scaffold, mediating interactions between RNA-binding proteins and transcriptional machinery, thereby influencing RNA processing, transport, and stability.
Studies highlight its involvement in muscle differentiation, osteogenesis, and tumorigenesis. FAM120B overexpression is linked to cancer progression (e.g., colorectal cancer and osteosarcoma), where it may promote cell proliferation, metastasis, and chemoresistance. In cardiovascular contexts, it regulates oxidative stress responses, potentially impacting atherosclerosis. Its paralog, FAM120A, shares functional redundancy in RNA metabolism, though distinct roles in disease pathways are emerging.
Recombinant FAM120B is typically produced in Escherichia coli or mammalian expression systems, purified for biochemical studies, structural analysis, and drug discovery. Its application spans investigating oxidative stress mechanisms, RNA-protein dynamics, and therapeutic target validation. Ongoing research aims to elucidate its precise molecular mechanisms, disease associations, and potential as a biomarker or intervention target in cancer and metabolic disorders.
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