| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM117A |
| Uniprot No | Q9C073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MAGAAAGGRGGGAWGPGRGGAGGLRRGCSPPAPAGSPRAGLQPLRATIPFQLQQPHQRRDGGGRAASVPCSVAPEKSVCRPQPLQVRRTFSLDTILSSYLLGQWPRDADGAFTCCTNDKATQTPLSWQELEGERASSCAHKRSASWGSTDHRKEISKLKQQLQRTKLSRSGKEKERGSPLLGDHAVRGALRASPPSFPSGSPVLRLSPCLHRSLEGLNQELEEVFVKEQGEEELLRILDIPDGHRAPAPPQSGSCDHPLLLLEPGNLASSPSMSLASPQPCGLASHEEHRGAAEELASTPNDKASSPGHPAFLEDGSPSPVLAFAASPRPNHSYIFKREPPEGCEKVRVFEEATSPGPDLAFLTSCPDKNKVHFNPTGSAFCPVNLMKPLFPGMGFIFRNCPSNPGSPLPPASPRPPPRKDPEASKASPLPFEPWQRTPPSEEPVLFQSSLMV |
| 分子量 | 74.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人FAM117A蛋白的虚构参考文献框架(实际需根据真实文献调整)：
1. **文献名称**：FAM117A regulates autophagy via mTOR signaling pathway
**作者**：Zhang et al., 2018
**摘要**：研究揭示了重组人FAM117A蛋白在调控细胞自噬中的作用，表明其通过与mTOR复合体相互作用影响自噬活性，可能在神经退行性疾病中具有潜在治疗价值。
2. **文献名称**：Expression and purification of recombinant human FAM117A in Escherichia coli
**作者**：Wang et al., 2020
**摘要**：报道了一种基于大肠杆菌的高效重组人FAM117A蛋白表达及纯化策略，并验证了其在体外结合DNA的能力，为后续功能研究提供了技术基础。
3. **文献名称**：FAM117A dysfunction links to Alzheimer’s disease pathology
**作者**：Chen et al., 2021
**摘要**：通过细胞模型证明，重组人FAM117A蛋白缺失会加剧β淀粉样蛋白毒性，提示其可能在阿尔茨海默病中通过调控氧化应激通路发挥保护作用。
4. **文献名称**：FAM117A interacts with β-catenin to modulate colon cancer progression
**作者**：Liu et al., 2022
**摘要**：研究发现重组人FAM117A蛋白通过结合β-catenin抑制Wnt信号通路，在结肠癌细胞中发挥抑癌功能，为靶向治疗提供了新靶点。
**注意**：以上文献为模拟示例，实际写作需替换为真实发表的论文(可通过PubMed、Google Scholar等平台检索)。FAM117A研究相对较少，建议结合相关家族蛋白或通路扩展文献范围。
Recombinant human FAM117A protein is a genetically engineered form of the FAM117A (family with sequence similarity 117 member A) protein, which is encoded by the FAM117A gene in humans. FAM117A is a conserved protein with homologs across species, but its precise molecular functions remain under investigation. Studies suggest its involvement in critical cellular processes, including maintenance of nuclear envelope integrity, regulation of endoplasmic reticulum (ER) stress response, and modulation of cell proliferation. Structurally, FAM117A contains a putative N-terminal transmembrane domain and intrinsically disordered regions, which may facilitate interactions with diverse partners such as nuclear membrane proteins or components of the unfolded protein response (UPR) pathway.
Emerging evidence links FAM117A dysregulation to pathological conditions. Downregulation of FAM117A has been observed in neurodegenerative disorders like Alzheimer’s disease, potentially influencing neuronal survival through ER stress pathways. Conversely, overexpression has been associated with tumorigenesis in certain cancers, hinting at context-dependent roles in disease. Recombinant FAM117A protein serves as a vital tool for mechanistic studies, enabling researchers to characterize its biochemical properties, post-translational modifications, and interactions in vitro. It also supports drug discovery efforts targeting FAM117A-related pathways. Further research is needed to fully elucidate its physiological roles and therapeutic potential.
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