| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM116B |
| Uniprot No | Q8NEG7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 氨基酸序列 | MDALLGTGPRRARGCLGAAGPTSSGRAARTPAAPWARFSAWLECVCVVTFDLELGQALELVYPNDFRLTDKEKSSICYLSFPDSHSGCLGDTQFSFRMRQCGGQRSPWHADDRHYNSRAPVALQREPAHYFGYVYFRQVKDSSVKRGYFQKSLVLVSRLPFVRLFQALLSLIAPEYFDKLAPCLEAVCSEIDQWPAPAPGQTLNLPVMGVVVQVRIPSRVDKSESSPPKQFDQENLLPAPVVLASVHELDLFRCFRPVLTHMQTLWELMLLGEPLLVLAPSPDVSSEMVLALTSCLQPLRFCCDFRPYFTIHDSEFKEFTTRTQAPPNVVLGVTNPFFIKTLQHWPHILRVGEPKMSGDLPKQVKLKKPSRLKTLDTKPGLYTAYTAHLHRDKALLKRLLKGVQKKRPSDVQSALLRRHLLELTQSFIIPLEHYMASLMPLQKSITPWKTPPQIQPFSQDDFLRSLEHAGPQLTCILKGDWLGLYRRFFKSPHFDGWYRQRHKEMALKLEALHLEAICEANIETWMKDKSEVEVVDLVLKLREKLVRAQGHQLPVKEATLQRAQLYIETVIGSLPKDLQAVLCPP |
| 分子量 | 92.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于真实文献的假设性示例(请注意，实际文献可能需要通过学术数据库验证)：
1. **文献名称**: "FAM116B regulates cell proliferation through the PI3K/AKT signaling pathway"
**作者**: Zhang L, et al.
**摘要**: 研究通过在大肠杆菌中表达重组人FAM116B蛋白，揭示其通过PI3K/AKT通路调控细胞增殖的作用，为肿瘤治疗提供潜在靶点。
2. **文献名称**: "Structural insights into FAM116B: A novel kinase-like domain involved in autophagy"
**作者**: Kim S, Park JH.
**摘要**: 利用重组表达的人FAM116B蛋白进行晶体结构解析，发现其具有激酶样结构域，并参与自噬调控的功能验证。
3. **文献名称**: "Recombinant FAM116B protein as a biomarker in colorectal cancer progression"
**作者**: Wang Y, et al.
**摘要**: 研究在结直肠癌组织中检测到FAM116B高表达，通过重组蛋白实验证明其与转移相关蛋白的相互作用及临床预后意义。
4. **文献名称**: "Functional characterization of FAM116B in DNA damage response using recombinant protein"
**作者**: Müller R, et al.
**摘要**: 使用昆虫细胞系统表达重组人FAM116B，证实其参与DNA损伤修复过程，并与ATM/ATR通路存在关联。
**备注**：以上文献为示例，实际研究中可能需要通过 **PubMed、Web of Science** 等平台检索关键词(如“FAM116B recombinant”或“TP53RK”)获取具体信息。建议结合最新数据库查询确认。
**Background of Recombinant Human FAM116B Protein**
The FAM116B protein, also known as TUBGCP4 (gamma-tubulin complex component 4), is a crucial regulator of microtubule nucleation and mitotic spindle assembly. It is part of the γ-tubulin ring complex (γ-TuRC), essential for organizing microtubule networks that maintain cell structure, division, and intracellular transport. FAM116B interacts with other γ-TuRC components to anchor microtubules to centrosomes, ensuring proper chromosomal segregation during mitosis. Dysregulation of FAM116B has been linked to cellular abnormalities, including mitotic errors, genomic instability, and ciliopathies, such as Joubert syndrome, a neurodevelopmental disorder associated with defective primary cilia.
Recombinant human FAM116B protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, molecular interactions, and role in disease mechanisms. Its recombinant form enables functional assays, structural studies (e.g., resolving coiled-coil domains mediating γ-TuRC assembly), and drug screening for therapies targeting microtubule-related disorders. Despite progress, FAM116B's precise regulatory mechanisms and tissue-specific functions remain under investigation, highlighting its potential as a therapeutic or diagnostic target in cancer and genetic diseases.
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