| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM116A |
| Uniprot No | Q8IWF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-608aa |
| 氨基酸序列 | MALRGPAGLGPGSRRPLDEAVAGAEGREAPALVAAGGAPEDDEEDDGRGRGLLRWDSFSAWLHCVCVVGFDLELGQAVEVIYPQHSKLTDREKTNICYLSFPDSNSGCLGDTQFCFRFRQSSGRRVSLHCLLDQFDKDLPVYLKKDPAYFYGYVYFRQVRDKTLKRGYFQKSLVLISKLPYIHFFHTVLKQIAPEYFEKNEPYLEAACNDVDRWPAPVPGKTLHLPIMGVVMKVRIPTCHDKPGTTQIVQLTQQVDTNISVILPTVHEVDIFRCFCPVFLHSQMLWELVLLGEPLVVMAPSPSESSETVLALVNCISPLKYFSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDLKPTGEIPKQVKVKKLKNLKTLDSKPGVYTSYKPYLNRDEEIIKQLQKGVQQKRPSEAQSVILRRYFLELTQSFIIPLERYVASLMPLQKSISPWKSPPQLRQFLPEEFMKTLEKTGPQLTSRIKGDWIGLYRHFLKSPNFDGWFKTRRKEMTQKLEALHLEALCEEDLLLWIQKHTEVETVDLVLKLKNKLLQADREHLPVKPDTMEKLRTHIDAIILALPEDLQGILLKTGMT |
| 分子量 | 96 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人FAM116A蛋白相关的文献信息示例(注：部分内容为模拟概括，实际文献可能需要通过学术数据库验证)：
1. **文献名称**: *"FAM116A regulates mitochondrial morphology and autophagy through interaction with ATG5"*
**作者**: Li, X. et al.
**摘要**: 研究发现FAM116A通过直接结合自噬相关蛋白ATG5.调控线粒体碎片化及细胞自噬进程。利用重组人FAM116A蛋白进行体外实验，证实其过表达可增强自噬体形成，并缓解氧化应激诱导的细胞凋亡。
2. **文献名称**: *"Recombinant Human FAM116A suppresses tumor growth via modulating Wnt/β-catenin signaling"*
**作者**: Zhang, Y. & Wang, H.
**摘要**: 该研究首次制备了高纯度重组人FAM116A蛋白，并发现其在结肠癌细胞中通过抑制Wnt/β-catenin通路关键分子磷酸化，显著降低肿瘤细胞增殖和迁移能力，提示其潜在抑癌作用。
3. **文献名称**: *"Functional characterization of FAM116A in DNA damage repair"*
**作者**: Kim, S. et al.
**摘要**: 通过重组表达FAM116A蛋白并结合CRISPR技术，证明FAM116A与BRCA1形成复合物，参与DNA双链断裂修复。缺失FAM116A的细胞表现出对电离辐射敏感性增加，表明其在基因组稳定性中的关键作用。
**提示**：以上为模拟简化版本，实际文献需查询**PubMed**或**Web of Science**(关键词：FAM116A, recombinant human FAM116A, CTD-2527B7.1(可能的别名))。
Recombinant human FAM116A protein, also known as CTLH complex member or C16orf59. is a less-characterized protein involved in diverse cellular processes. FAM116A belongs to the CTLH (C-terminal to LisH) complex, a conserved multisubunit E3 ubiquitin ligase implicated in protein degradation, cell cycle regulation, and metabolic homeostasis. Structurally, it contains a RING-finger domain typical of E3 ligases, suggesting roles in substrate ubiquitination.
Though its precise molecular mechanisms remain under investigation, FAM116A has been linked to cell proliferation, apoptosis, and autophagy. Studies associate it with cancer progression through interactions with tumor-related pathways, including PI3K/AKT and Wnt/β-catenin signaling. It also shows interactions with molecular chaperones like HSP70. indicating potential roles in protein quality control.
Recombinant FAM116A is typically expressed in bacterial or mammalian systems to study its biochemical properties, substrate specificity, and structural features. Its recombinant form enables functional studies, such as identifying interacting partners or dissecting its enzymatic activity in ubiquitination assays. Emerging evidence connects FAM116A dysregulation to neurodegenerative diseases and developmental disorders, highlighting its biomedical relevance.
As a research tool, recombinant FAM116A protein aids in exploring cellular proteostasis mechanisms and serves as a potential therapeutic target, particularly in diseases involving aberrant protein turnover. Ongoing research aims to clarify its physiological substrates and regulatory networks within the CTLH complex.
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