| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM115A |
| Uniprot No | Q9Y4C2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-921aa |
| 氨基酸序列 | MATPSAAFEALMNGVTSWDVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLVVVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPIGVHPSLAPLAKILEGSGVDAKVEPEVKDSLGVYCIDAYNETMTEKLVKFMKCGGGLLIGGQAWDWANQGEDERVLFTFPGNLVTSVAGIYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSDDREELLHGISELDISNSDCFPSQLLVHGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGKIVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSEVGVKELQEFVAEGGGLFVGAQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCTNPGTRYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPTKSITCLWGGLLYIIVPQNSKLGSVPVTVKGAVHAPYYKLGETTLEEWKRRIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVREKRVRIYLSKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTENVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQMPH |
| 分子量 | 128.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为虚构示例的3篇重组人FAM115A蛋白相关参考文献(实际文献需通过数据库查询验证)：
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1. **文献名称**: "Recombinant human FAM115A protein inhibits cancer cell proliferation via modulating cell cycle regulators"
**作者**: Li, H., Wang, J., & Chen, Z.
**摘要**: 本研究报道了重组人FAM115A蛋白在体外通过上调p21和下调Cyclin D1表达，阻滞肿瘤细胞G1/S期转换，抑制结肠癌细胞增殖。
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2. **文献名称**: "Structural characterization of FAM115A and its interaction with deubiquitinase USP7"
**作者**: Smith, R.K., et al.
**摘要**: 通过X射线晶体学解析重组FAM115A蛋白结构，发现其C端结构域与去泛素化酶USP7结合，提示其可能参与泛素-蛋白酶体调控通路。
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3. **文献名称**: "FAM115A as a novel tumor suppressor in renal cell carcinoma: Role of recombinant protein in apoptosis induction"
**作者**: Zhang, Y., et al.
**摘要**: 实验证实重组FAM115A蛋白可激活肾癌细胞线粒体凋亡途径，通过Bax/Bcl-2比例失衡及Caspase-3活化诱导细胞凋亡，提示其抑癌潜力。
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如需真实文献，建议使用以下数据库关键词检索：
- **PubMed/Google Scholar关键词**:
`"recombinant FAM115A"` / `"FAM115A protein function"` / `"FAM115A AND (cancer OR cell cycle)"`
Recombinant human FAM115A protein is a genetically engineered form of the FAM115A (family with sequence similarity 115 member A) protein, encoded by the FAM115A gene located on human chromosome 7. As a member of the FAM115 family, this protein is evolutionarily conserved and ubiquitously expressed, particularly in tissues like the brain, liver, and kidneys. Structurally, it contains a conserved KY domain, suggesting potential roles in protein-protein interactions or nucleic acid binding, though its precise molecular functions remain under investigation.
Emerging studies link FAM115A to cellular processes such as transcriptional regulation, stress response, and cell proliferation. Dysregulation of FAM115A has been implicated in pathological conditions, including certain cancers (e.g., hepatocellular carcinoma) and neurodegenerative disorders, highlighting its potential as a therapeutic target. However, mechanistic insights are limited due to challenges in isolating endogenous FAM115A and its low abundance in native tissues.
The recombinant protein, typically produced in bacterial or mammalian expression systems, enables biochemical and functional studies. It serves as a critical tool to explore FAM115A's interactome, enzymatic properties (if any), and its influence on signaling pathways like Wnt/β-catenin or MAPK. Ongoing research aims to clarify its role in disease pathogenesis and assess its utility in diagnostics or targeted therapies.
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