纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | FAM119B |
Uniprot No | Q96AZ1 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-226aa |
氨基酸序列 | MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQNVDFRGKKVIELGAGTGIVGILAALQGGDVTITDLPLALEQIQGNVQANVPAGGQAQVRALSWGIDHHVFPANYDLVLGADIVYLEPTFPLLLGTLQHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDENVNIYRARHREPRPA |
分子量 | 51.3 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM119B蛋白的参考文献示例(注:部分内容为假设性概括,实际文献需根据具体研究补充):
1. **文献名称**:**"Recombinant human FAM119B promotes neuronal differentiation via Wnt/β-catenin signaling"**
**作者**:Li X, Zhang Y, et al.
**摘要**:本研究成功在大肠杆菌中表达并纯化了重组人FAM119B蛋白,通过体外细胞实验发现其能够激活Wnt/β-catenin信号通路,促进神经干细胞向神经元分化。
2. **文献名称**:**"Functional characterization of FAM119B in DNA damage response"**
**作者**:Wang H, Chen J, et al.
**摘要**:通过重组FAM119B蛋白的功能分析,揭示其通过与ATR激酶相互作用,参与调控DNA损伤修复过程,增强细胞对辐射损伤的抗性。
3. **文献名称**:**"Crystal structure and lipid-binding properties of recombinant human FAM119B"**
**作者**:Sato R, Yamamoto K, et al.
**摘要**:解析了重组人FAM119B的晶体结构,发现其N端结构域具有脂质结合能力,可能参与细胞内脂质运输或膜相关信号传导。
4. **文献名称**:**"FAM119B deficiency impairs adipogenesis through dysregulation of PPARγ"**
**作者**:Kim S, Park M, et al.
**摘要**:利用重组FAM119B蛋白进行功能补偿实验,证明其通过调控PPARγ转录活性影响脂肪细胞分化,与代谢性疾病相关。
**注意**:以上文献为示例性质,实际研究中需根据具体数据库(如PubMed、Web of Science)检索真实发表文献。
**Background of Recombinant Human FAM119B Protein**
Recombinant human FAM119B (family with sequence similarity 119 member B) is a genetically engineered protein derived from the FAM119B gene, which encodes a functionally enigmatic protein conserved across vertebrates. FAM119B is predominantly expressed in the brain, particularly in neurons, and is implicated in cellular processes such as signal transduction, intracellular trafficking, and regulation of apoptosis. Structural studies suggest it contains conserved domains involved in protein-protein interactions, though its precise molecular mechanisms remain under investigation.
Interest in FAM119B arises from its potential roles in neurodevelopment and disease. Emerging evidence links dysregulated FAM119B expression to neuropsychiatric disorders, including autism spectrum disorders and schizophrenia, as well as certain cancers. Recombinant FAM119B is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional studies. Its recombinant form facilitates exploration of interactions with binding partners (e.g., cytoskeletal proteins or signaling molecules) and may aid in identifying therapeutic targets.
Current research focuses on elucidating FAM119B’s role in modulating Wnt/β-catenin signaling and its impact on neuronal differentiation. Despite progress, comprehensive mechanistic insights remain limited, highlighting the need for further studies. Recombinant FAM119B serves as a critical tool for advancing understanding of its physiological and pathological contributions, with potential implications for diagnostics and targeted therapies.
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