| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM125B |
| Uniprot No | Q9H7P6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MRSCFCVRRS RDPPPPQPPP PPPQRGTDQS TMPEVKDLSE ALPETSMDPI TGVGVVASRN RAPTGYDVVA QTADGVDADL WKDGLFKSKV TRYLCFTRSF SKENSHLGNV LVDMKLIDIK DTLPVGFIPI QETVDTQEVA FRKKRLCIKF IPRDSTEAAI CDIRIMGRTK QAPPQYTFIG ELNSMGIWYR MGRVPRNHDS SQPTTPSQSS AASTPAPNLP RHISLTLPAT FRGRNSTRTD YEYQHSNLYA ISAMDGVPFM ISEKFSCVPE SMQPFDLLGI TIKSLAEIEK EYEYSFRTEQ SAAARLPPSP TRCQQIPQS |
| 分子量 | 50.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM125B蛋白的示例参考文献(注：由于实际文献可能有限，以下为假设性示例，建议通过学术数据库检索真实文献)：
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1. **"Cloning and Characterization of Recombinant Human FAM125B Protein in E. coli"**
*Authors: Zhang L, Wang Y, Chen X*
摘要：本研究通过在大肠杆菌系统中成功表达并纯化了重组人FAM125B蛋白，分析表明该蛋白在体外可稳定结合微管结构，揭示了其在细胞骨架动态调控中的潜在作用。
2. **"Structural Insights into FAM125B and Its Role in Neuronal Development"**
*Authors: Smith J, Tanaka K, Lee S*
摘要：利用重组人FAM125B蛋白进行X射线晶体学分析，发现其C端结构域对神经元轴突生长至关重要，并通过敲低实验验证了其在小鼠神经发育中的功能缺失表型。
3. **"FAM125B Interaction with PI3K-AKT Signaling Pathway in Cancer Cells"**
*Authors: Gupta R, Martinez F*
摘要：通过重组FAM125B蛋白的免疫共沉淀实验，发现其与PI3K激酶复合物存在直接相互作用，可能通过调控AKT磷酸化影响肿瘤细胞迁移和增殖。
4. **"Development of a Monoclonal Antibody Specific to Recombinant FAM125B for Diagnostic Applications"**
*Authors: Kim H, Park D, Ishikawa T*
摘要：基于重组人FAM125B蛋白制备出高特异性单克隆抗体，应用于结直肠癌组织样本检测，显示FAM125B表达水平与患者生存率显著相关。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science或Google Scholar检索真实文献(关键词：*FAM125B*、*KIAA1720*、*recombinant expression*)。如相关研究较少，可扩展至该蛋白的别名(如C1orf123)或功能相关领域文献。
**Background of Recombinant Human FAM125B Protein**
Recombinant human FAM125B protein, encoded by the *FAM125B* gene (also known as *KNDC1*), is a biologically engineered protein produced using recombinant DNA technology. This gene, located on chromosome 12. is conserved across species and primarily expressed in the brain and testis. FAM125B contains a C-terminal Ras-association (RA) domain and an N-terminal kinase non-catalytic C-lobe (KIND) domain, suggesting roles in signaling pathways and protein-protein interactions.
FAM125B interacts with Ras-related GTPases and cytoskeletal regulators, influencing cell migration, differentiation, and neural development. Studies link it to modulating the mTOR pathway and actin dynamics, critical for synaptic plasticity and neuronal morphology. Dysregulation of FAM125B is implicated in neurodevelopmental disorders, intellectual disability, and cancers like glioblastoma.
The recombinant protein is generated by cloning the *FAM125B* gene into expression vectors (e.g., bacterial or mammalian systems), followed by purification for experimental use. It serves as a tool to study cellular signaling, neuronal function, and disease mechanisms, with potential applications in therapeutic target discovery. Its recombinant form ensures high purity and consistency, enabling precise biochemical, structural, and functional analyses in research settings.
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