| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM122B |
| Uniprot No | Q7Z309 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MAQEKMELDLEPDTSYGGTLRRSSSAPLIHGLSDLSQVFQPYTLRTRRNSTTIMSRHSLEEGLDMVNRETAHEREMQTAMQISQSWDESLSLSDSDFDKPEKLYSPKRIDFTPVSPAPSPTRGFGKMFVSSSGLPPSPVPSPRRFSSRRSQSPVKCIRPSVLGPLKRKGEMETESQPKRLFQGTTNMLSPDAAQLSDLSSCSDILDGSSSSSGLSSDPLAKGSATAESPVACSNSCSSFILMDDLSPK |
| 分子量 | 53.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM122B蛋白的示例参考文献(虚拟示例，供参考)：
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1. **标题**: "Expression, purification, and functional characterization of recombinant human FAM122B in Escherichia coli"
**作者**: Zhang Y et al.
**摘要**: 研究报道了人源FAM122B蛋白在大肠杆菌中的重组表达和纯化，优化了可溶性表达条件，并通过体外实验证明其与磷酸酶PP2A的相互作用，提示其在细胞周期调控中的潜在作用。
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2. **标题**: "Structural insights into FAM122B reveals a novel regulator of PP2A phosphatase activity"
**作者**: Li X et al.
**摘要**: 通过X射线晶体学解析重组人FAM122B的三维结构，发现其通过特定结构域结合PP2A催化亚基，抑制其磷酸酶活性，为肿瘤发生中FAM122B的功能研究提供分子基础。
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3. **标题**: "FAM122B knockdown promotes apoptosis via PP2A-mediated signaling in hepatocellular carcinoma"
**作者**: Wang L et al.
**摘要**: 利用重组FAM122B蛋白进行体外功能实验，发现其通过调控PP2A活性影响肝癌细胞凋亡通路，表明FAM122B可能作为癌症治疗的潜在靶点。
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4. **标题**: "Proteomic analysis identifies FAM122B as a p53-regulated gene involved in DNA damage response"
**作者**: Chen S et al.
**摘要**: 研究发现重组FAM122B在DNA损伤条件下表达上调，可能通过稳定p53蛋白参与细胞应激反应，揭示了其在基因组稳定性中的作用机制。
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**注意**：以上文献为虚拟示例，实际研究请通过PubMed、Web of Science或Google Scholar等数据库检索关键词“recombinant FAM122B”或“FAM122B protein”获取。
FAM122B (Family with sequence similarity 122B) is a conserved nuclear protein encoded by the FAM122B gene in humans, though its biological functions remain incompletely characterized. Structurally, it contains predicted phosphorylation sites and intrinsically disordered regions, suggesting roles in protein interactions and signaling modulation. Studies indicate FAM122B may act as a regulatory component of the protein phosphatase 2A (PP2A) complex, influencing cell cycle progression and apoptosis. While its paralog FAM122A is better studied as a cell growth suppressor by inhibiting Akt phosphorylation and ERK signaling, FAM122B's specific mechanisms are less defined.
Emerging evidence links FAM122B to cancer biology. It is downregulated in hepatocellular carcinoma, correlating with poor prognosis, and may suppress tumorigenesis by enhancing PP2A activity. Conversely, FAM122B overexpression in gliomas promotes cell proliferation, implying context-dependent roles. Recombinant human FAM122B protein is typically produced in E. coli or mammalian systems, enabling in vitro studies on its biochemical properties, interactome (e.g., PP2A subunits), and enzymatic regulation. Current research focuses on resolving its structural dynamics via X-ray crystallography or cryo-EM and clarifying dual roles in tumor suppression/oncogenesis. Despite limited functional data, FAM122B represents a potential therapeutic target, warranting further exploration of its post-translational modifications and tissue-specific signaling networks.
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