| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM127A |
| Uniprot No | A6ZKI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MGGGRGLLGRETLGPGGGCSGEGPLCYWPPPGSPPAPSLRASLPLEPPRCPLRSCSLPRSACLCSRNSAPGSCCRPWASLWSEPPPSPSSQPAPPMYIWTLSCAPAASWAPVTHWTDHPLPPLPSPLLPTRLPDDYIILPTDLRCHSHRHPSHPTDRLLLLVIWTHLGGIWAGHSPWTVIQTAGRPPRDLSPSARPISSPPPETSCVLA |
| 分子量 | 48.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于文献特征的模拟示例(请注意，部分内容可能需要通过实际数据库验证)：
1. **《FAM127A interacts with COP1 and regulates cell proliferation in breast cancer cells》**
- 作者：Zhang, Y. et al.
- 摘要：研究利用重组人FAM127A蛋白揭示其通过结合COP1蛋白调控乳腺癌细胞周期和增殖的分子机制，提示其潜在抑癌功能。
2. **《Cloning and functional characterization of recombinant human FAM127A in neuronal differentiation》**
- 作者：Li, X. & Wang, H.
- 摘要：报道在大肠杆菌和哺乳动物细胞中成功表达重组hFAM127A，发现其促进神经细胞分化，可能与MAPK通路激活相关。
3. **《Structural insights into the FAM127A-PH domain and its interaction with phosphoinositides》**
- 作者：Garcia-Saez, I. et al.
- 摘要：通过重组蛋白结晶解析FAM127A的PH结构域三维结构，揭示其特异性结合磷脂酰肌醇的分子基础，为功能研究提供依据。
4. **《FAM127A deficiency promotes mitochondrial dysfunction and oxidative stress in hepatocellular carcinoma》**
- 作者：Chen, L. et al.
- 摘要：利用重组FAM127A体外回补实验证明其通过抑制ROS积累调控肝癌细胞线粒体稳态，与患者预后相关。
**注意**：以上为假设性示例，实际文献需通过PubMed、Google Scholar等平台以关键词"recombinant human FAM127A"或"FAM127A function"检索核实。
FAM127A (Family with sequence similarity 127 member A) is a human gene encoding a protein implicated in diverse cellular processes, though its exact biological functions remain incompletely characterized. Initially identified through genomic studies, FAM127A has drawn attention due to its potential role in carcinogenesis. Emerging evidence suggests its involvement in regulating cell proliferation and apoptosis, possibly through interactions with key signaling pathways such as RAS/RAF/MEK/ERK. Structural analysis reveals conserved domains that may mediate protein-protein interactions, hinting at its role as a regulatory component in signaling networks.
The recombinant human FAM127A protein, produced via heterologous expression systems like *E. coli* or mammalian cell cultures, enables mechanistic studies on its molecular functions. Purified recombinant variants (wild-type or mutated) are crucial for *in vitro* assays, antibody development, and drug screening. Notably, FAM127A overexpression has been associated with tumor progression in cervical and breast cancers, while epigenetic silencing is observed in other malignancies, suggesting context-dependent roles in oncogenesis.
Current research focuses on elucidating FAM127A’s interplay with tumor suppressors, oncoproteins, and non-coding RNAs. Its dual roles in different cancer types highlight therapeutic potential as a biomarker or intervention target. However, comprehensive mechanistic insights and *in vivo* validation are required to clarify its pathophysiological relevance and translational applications.
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