关键信息
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基因名
TSFM
- 应用
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别名
TSFM；Elongation factor Ts. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43897-2
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表达区间
46-346aa
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氨基酸序列
MSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKA AKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALG TMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLP AGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQS PSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDD EPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAE TE
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of TSFM (TSF mitochondrial translation factor) recombinant protein is rooted in the broader understanding of mitochondrial function and protein synthesis within cells. Mitochondria, often referred to as the powerhouses of the cell, are essential for energy production through oxidative phosphorylation. However, their function relies heavily on the accurate translation of mitochondrial-encoded proteins, which is where TSFM plays a vital role. It is involved in the mitochondrial translation process, aiding in the proper folding and assembly of mitochondrial ribosomes. Dysregulation or mutations in TSFM can lead to mitochondrial dysfunction, which has been linked to various diseases, including neurodegenerative disorders and metabolic syndromes. Research into TSFM recombinant protein not only provides insights into the fundamental mechanisms of mitochondrial translation but also paves the way for therapeutic approaches targeting mitochondrial pathologies. By producing and studying recombinant TSFM protein, researchers can investigate its structure, function, and interactions with other mitochondrial components, contributing to a deeper understanding of mitochondrial biology and its implications for human health.
