| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM133A |
| Uniprot No | Q8N9E0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MGKRDNRVAYMNPIAMARWRGPTQSVGPTIQDYLNRPRPTWEEVKKQLENKKTGSKALAEFEEKMNENWKKELEKSREKLLSGNESSSKKRERKKKRKKKSCRSSSSSSSSDSSSSSSDSEDEEKKQGKRRKKKKNRSYKSSQSSTHESESESKESVKKKKKSKDETEKEKDVRSLSKKRKKSYPDDKPLSSESSSESDYEEDVQAKKKRRCEEREQAKEKVKKKKKKQHKKHSKKKKKKSGSSHKSR |
| 分子量 | 55.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于“重组人FAM133A蛋白”的示例参考文献列表(注：由于FAM133A的研究较为冷门且部分信息可能存在时效性，以下内容根据推测整理，建议核实具体文献)：
**1. 文献名称**：**《FAM133A interacts with HOXB4 to promote neuronal differentiation》**
**作者**：Zhang Y, et al.
**摘要**：本研究首次成功在大肠杆菌系统中表达并纯化了重组人FAM133A蛋白，发现其通过与转录因子HOXB4相互作用，调控神经干细胞的分化过程，暗示其在神经发育中的潜在作用。
**2. 文献名称**：**《Structural and functional analysis of recombinant human FAM133A in antiviral innate immunity》**
**作者**：Wang L, et al.
**摘要**：通过哺乳动物表达系统获得重组人FAM133A蛋白，结合质谱分析发现其与RIG-I信号通路相关蛋白结合，可能通过增强I型干扰素应答抑制病毒复制，为抗病毒治疗提供新靶点。
**3. 文献名称**：**《Recombinant FAM133A as a biomarker for glioblastoma progression》**
**作者**：Chen X, et al.
**摘要**：研究利用重组FAM133A蛋白制备单克隆抗体，发现其在胶质母细胞瘤患者血清中表达水平升高，并与肿瘤侵袭性相关，提示其作为预后标志物的潜力。
**4. 文献名称**：**《FAM133A regulates mitochondrial dynamics through binding to Drp1》**
**作者**：Kim S, et al.
**摘要**：通过真核表达系统获得重组FAM133A蛋白，发现其直接结合线粒体分裂蛋白Drp1.调控线粒体形态和细胞凋亡，可能在神经退行性疾病中发挥作用。
**说明**：以上文献为模拟内容，实际研究可能存在差异。建议通过**PubMed、Google Scholar**等平台以关键词“FAM133A”、“recombinant FAM133A”检索最新进展。
**Background of Recombinant Human FAM133A Protein**
Recombinant human FAM133A (family with sequence similarity 133 member A), also known as CMT2Z or CIP2A-interacting protein, is a relatively uncharacterized protein implicated in cellular processes such as neuronal development, cytoskeletal regulation, and stress response. Initially identified through genomic studies, FAM133A gained attention due to its association with Charcot-Marie-Tooth disease type 2Z (CMT2Z), a hereditary neuropathy linked to mutations in the *FAM133A* gene. The protein is hypothesized to interact with cytoskeletal components, particularly β-tubulin, influencing microtubule dynamics critical for axonal maintenance in neurons.
Structurally, FAM133A contains conserved domains predicted to mediate protein-protein interactions, though detailed mechanistic insights remain limited. Recombinant production of FAM133A in bacterial or mammalian expression systems enables functional studies, including its role in cellular stress adaptation and potential involvement in neurodegenerative or cancer pathways. Emerging evidence suggests it may modulate cell survival under oxidative or hypoxic conditions, highlighting its broader relevance in disease contexts.
Current research focuses on clarifying FAM133A's molecular interactions, post-translational modifications, and tissue-specific functions, aiming to unravel its therapeutic potential as a biomarker or target for neuropathies and other disorders.
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