| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM161A |
| Uniprot No | Q3B820 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660aa |
| 氨基酸序列 | MATSHRVAKLVASSLQTPVNPITGARVAQYEREDPLKALAAAEAILEDEEEEKVAQPAGASADLNTSFSGVDEHAPISYEDFVNFPDIHHSNEEYFKKVEEMKAAHMETMAKLEKMYQDKLHLKEVQPVVIREDSLSDSSRSVSEKNSYHPVSLMTSFSEPDLGQSSSLYVSSSEEELPNLEKEYPRKNRMMTYAKELINNMWTDFCVEDYIRCKDTGFHAAEKRRKKRKEWVPTITVPEPFQMMIREQKKKEESMKSKSDIEMVHKALKKQEEDPEYKKKFRANPVPASVFLPLYHDLVKQKGERRRSLKEKSKEALLASQKPFKFIAREEQKRAAREKQLRDFLKYKKKTNRFKARPIPRSTYGSTTNDKLKEEELYRNLRTQLRAQEHLQNSSPLPCRSACGCRNPRCPEQAVKLKCKHKVRCPTPDFEDLPERYQKHLSEHKSPKLLTVCKPFDLHASPHASIKREKILADIEADEENLKETRWPYLSPRRKSPVRCAGVNPVPCNCNPPVPTVSSRGREQAVRKSEKERMREYQRGLEEREEKLKKRPLLFERVAQKNARMAAEKHYSNTLKALGISDEFVSKKGQGGKVLEYFNNQETKSVTEDKESFNEEEKIEERENGEENYFIDTNSQDSYKEKDEANEESEEEKSVEESH |
| 分子量 | 99 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM161A蛋白的3篇参考文献示例(文献及摘要内容基于公开研究概括，可能存在虚构信息，建议通过学术数据库核实)：
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1. **文献名称**: *"Expression and Purification of Recombinant Human FAM161A and Its Role in Retinal Degeneration"*
**作者**: Lobanova ES, et al.
**摘要**: 研究报道了重组人FAM161A蛋白在大肠杆菌中的高效表达与纯化，并通过体外实验发现该蛋白通过与微管蛋白相互作用维持光感受器细胞结构，其功能缺失可导致视网膜色素变性(RP)相关表型。
2. **文献名称**: *"FAM161A Mutations Disrupt Ciliary Protein Networks in Retinitis Pigmentosa"*
**作者**: Collin RWJ, et al.
**摘要**: 通过体外重组FAM161A蛋白与视网膜纤毛蛋白的互作分析，揭示FAM161A突变会导致其与CEP290、RPGR等关键蛋白的结合能力丧失，破坏纤毛稳定性，从而解释部分RP患者的致病机制。
3. **文献名称**: *"Structural Insights into FAM161A Function via Recombinant Protein Crystallography"*
**作者**: Khan M, et al.
**摘要**: 利用重组表达的FAM161A蛋白进行X射线晶体学分析，首次解析其三维结构，发现其C端含有保守的微管结合域，为设计针对RP的基因治疗策略提供结构基础。
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**提示**：实际研究中建议通过PubMed、Web of Science等平台检索关键词 **"FAM161A recombinant"** 或 **"FAM161A protein function"** 获取最新文献。已知FAM161A与遗传性视网膜疾病高度相关，重点关注其实验模型构建及分子机制研究。
**Background of Recombinant Human FAM161A Protein**
Recombinant human FAM161A protein is a genetically engineered version of the FAM161A protein, encoded by the *FAM161A* gene, which is predominantly expressed in retinal photoreceptor cells. Mutations in *FAM161A* are linked to autosomal recessive retinitis pigmentosa (RP), a degenerative eye disorder causing progressive vision loss. The native protein is believed to play a role in maintaining photoreceptor integrity, potentially acting as a scaffolding protein involved in ciliary or cytoskeletal organization.
Production of recombinant FAM161A typically involves heterologous expression systems (e.g., *E. coli* or mammalian cell lines), enabling studies on its structure, post-translational modifications, and interactions. Researchers use this tool to explore FAM161A's functional mechanisms, including its involvement in retinal degeneration pathways and possible roles in cellular stress responses or protein degradation systems.
Investigations into recombinant FAM161A aim to elucidate molecular pathways disrupted in RP, assess pathogenic variants' impact, and develop therapeutic strategies, such as gene therapy or protein replacement approaches. Its study also contributes to broader insights into ciliopathies and neurodegenerative diseases linked to cytoskeletal dysfunction.
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