| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM181A |
| Uniprot No | Q8N9Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MPLEERRSSG ERNDAAPTNH RRPGEKRAST AKQVSSVPFL GAAGHQQSLP SSWKASCSGP LVMASDSDVK MLLNFVNLAS SDIKAALDKS APCRRSVDHR KYLQKQLKRF SQKYSRLPRG LPGRAAEPYL KRGSEDRPRR LLLDLGPDSS PGGGGGCKEK VLRNPYREEC LAKEQLPQRQ HPEAAQPGQV PMRKRQLPAS FWEEPRPTHS YHVGLEGGLG PREGPPYEGK KNCKGLEPLG PETTLVSMSP RALAEKEPLK MPGVSLVGRV NAWSCCPFQY HGQPIYPGPL GALPQSPVPS LGLWRKSPAF PGELAHLCKD VDGLGQKVCR PVVLKPIPTK PAVPPPIFNV FGYL |
| 分子量 | 38.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM181A蛋白的假设性参考文献示例(请注意，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*Expression and Functional Analysis of Recombinant Human FAM181A in Germ Cell Development*
**作者**：Li, X. et al. (2019)
**摘要**：该研究成功表达并纯化了重组人FAM181A蛋白，发现其在睾丸组织特异性高表达，可能通过调控细胞周期蛋白参与精子生成过程，体外实验表明该蛋白促进生殖细胞系的增殖。
2. **文献名称**：*FAM181A Regulates Cancer Cell Proliferation via MAPK Signaling Pathway*
**作者**：Zhang, Y. et al. (2020)
**摘要**：利用重组FAM181A蛋白进行体外功能研究，发现其通过抑制MAPK通路抑制肺癌细胞增殖，提示其在癌症治疗中具有潜在应用价值。
3. **文献名称**：*Structural Characterization of Recombinant FAM181A and Its Nuclear Localization Mechanism*
**作者**：Wang, Q. et al. (2021)
**摘要**：通过重组蛋白技术解析FAM181A的晶体结构，揭示了其核定位信号(NLS)的关键结构域，并证实该蛋白通过调控基因转录影响细胞分化。
4. **文献名称**：*FAM181A as a Potential Biomarker for Male Infertility*
**作者**：Patel, R. et al. (2022)
**摘要**：研究通过重组FAM181A蛋白开发ELISA检测方法，发现其在少精症患者血清中显著低表达，可能成为诊断男性不育的生物标志物。
---
**注意事项**：
- 上述文献为示例性内容，实际研究需查阅PubMed、Web of Science等数据库。
- 若需具体文献，可提供关键词或DOI协助检索。
**Recombinant Human FAM181A Protein: Background Overview**
FAM181A (Family with sequence similarity 181 member A) is a conserved but functionally enigmatic protein encoded by the *FAM181A* gene in humans. While its exact biological roles remain unclear, emerging studies suggest potential involvement in cellular processes such as cell cycle regulation, spermatogenesis, and neuronal development. The gene is expressed across various tissues, including the brain, testis, and placenta, hinting at diverse physiological or pathophysiological roles.
Interest in recombinant human FAM181A protein stems from the need to elucidate its molecular mechanisms. Recombinant versions are typically produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its structure, interactions, and functions in vitro. These studies aim to clarify its binding partners (e.g., microtubules or chromatin-associated proteins) and its possible role in diseases, such as infertility or neurodevelopmental disorders.
Current challenges include limited functional data and conflicting reports about its subcellular localization (nuclear vs. cytoplasmic). Recombinant FAM181A serves as a critical tool to address these gaps, facilitating antibody generation, structural analysis, and pathway mapping. Future research may explore its therapeutic potential or diagnostic relevance as mechanistic insights deepen. Overall, recombinant FAM181A represents a gateway to decoding a protein with broad, yet undefined, significance in human biology.
×
