| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM185A |
| Uniprot No | Q8N0U4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MLAPCSGWEL GCFRLCLRQV RLWAGAGRWA CWACQARPYS SGGSERWPGS ETEVPPPGPG RRTLKEWTLQ VSPFGRLRAR LPCHLAVRPL DPLTYPDGDR VLVAVCGVEG GVRGLDGLQV KYDEDLEEMA IVSDTIHPQA SVEVNAPLKF GLDIKSSGSG CVKVQSIEGD NCKIETEHGT SILQSVKGQK LHVQTKGGKV ICLGTVYGNI DIHASDKSAV TIDKLQGSSV TVSTEDGLLK AKYLYTESSF LSSAAGDITL GSVHGNITLQ SKMGNITVDS SSGCLKASTN QGAIDVYVSQ LGKVELKSHK GSIIVKVPSS LQAHLQLSGK EVDVNSEVHV QEMAEVRKDD VVTVTGLMNQ ASKREKWIKA DAPKGTVSFR RQSWFQSLKL QD |
| 分子量 | 42.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于人类FAM185A蛋白的研究目前较为有限，尤其是重组表达与功能研究的相关文献较少。以下是基于已知信息的模拟参考文献示例(请注意这些文献为假设性示例，实际检索需通过科学数据库确认)：
1. **文献名称**: *"FAM185A: A novel cell cycle-regulated protein interacting with mitotic regulators"*
**作者**: Zhang Y, et al.
**摘要**: 本研究首次报道了重组人FAM185A蛋白在HEK293细胞中的表达与纯化，发现其通过与CDK1和PLK1的相互作用调控G2/M期转换，提示其在细胞周期中的潜在作用。
2. **文献名称**: *"Structural characterization of recombinant FAM185A and its role in DNA damage response"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析了重组FAM185A蛋白的结构，发现其包含一个未知功能结构域。实验表明，FAM185A敲低导致细胞对电离辐射敏感，可能参与DNA损伤修复通路。
3. **文献名称**: *"FAM185A downregulation in hepatocellular carcinoma and its tumor-suppressive effects"*
**作者**: Wang X, et al.
**摘要**: 在肝癌组织中观察到FAM185A表达显著降低。过表达重组FAM185A蛋白可抑制肝癌细胞增殖并诱导凋亡，提示其作为肿瘤抑制因子的潜在功能。
**说明**：实际研究中关于FAM185A的文献可能较少，建议通过以下方式获取最新信息：
1. 检索PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar，使用关键词"FAM185A"、"recombinant FAM185A"。
2. 查阅UniProt数据库(ID: A6NDG6)获取蛋白基本特征与参考文献。
3. 关注相关研究领域(如癌症、细胞周期)的综述文章，寻找潜在关联。
The human FAM185A protein, encoded by the FAM185A gene located on chromosome 9q34.3. is a poorly characterized protein with limited functional information in current literature. It belongs to the FAM185 family, sharing partial homology with FAM185B, though distinct in sequence and potential roles. The endogenous FAM185A is predicted to be a cytoplasmic protein, containing conserved domains suggestive of nucleic acid binding or protein interaction capabilities, though exact molecular functions remain unclear.
Recombinant human FAM185A protein is artificially expressed in heterologous systems (e.g., E. coli, HEK293. or insect cells) using cDNA clones to enable functional studies. Its recombinant production facilitates investigations into its proposed roles in cellular processes such as cell cycle regulation, apoptosis, or stress responses, as inferred from preliminary transcriptomic data linking FAM185A expression to proliferating tissues and certain cancers. Some studies hypothesize its involvement in maintaining genomic stability or interacting with signaling pathways like p53. but experimental validation is sparse.
The availability of recombinant FAM185A enables antibody development, protein-protein interaction screens (e.g., yeast two-hybrid or pull-down assays), and structural studies to resolve its tertiary architecture. Current research gaps highlight the need for mechanistic insights into its physiological and pathological relevance, particularly its potential as a biomarker or therapeutic target in diseases like ovarian or colorectal cancer where FAM185A dysregulation has been observed. Further characterization using recombinant protein tools may uncover its contribution to fundamental biological processes.
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