| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM21C |
| Uniprot No | Q9Y4E1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ |
| 分子量 | 56.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM21C蛋白的3篇参考文献(示例为虚构，仅供格式参考)：
1. **名称**：《WASH复合体调控内吞循环的分子机制》
**作者**：Derivery E. et al.
**摘要**：研究揭示了FAM21C作为WASH复合体核心组分的关键作用，通过调控肌动蛋白成核促进内吞小体的膜重塑及货物分选，揭示了其与溶酶体降解途径的关联。
2. **名称**：《FAM21C羧基末端结构域与逆向膜运输的关联》
**作者**：Jia D. et al.
**摘要**：通过冷冻电镜解析FAM21C羧基末端多螺旋结构域，阐明其与Retromer复合体的相互作用机制，为神经退行性疾病相关突变(如VPS35)提供分子基础。
3. **名称**：《FAM21C在阿尔茨海默病模型中的异常表达及功能缺失》
**作者**：Simonetti B. et al.
**摘要**：发现FAM21C表达异常导致β-淀粉样蛋白积累，证明其通过WASH复合体调控APP蛋白运输，为阿尔茨海默病的病理机制提供新视角。
(注：以上文献为示例性概括，实际文献需通过PubMed/Google Scholar检索关键词“FAM21C”“WASH complex”“retromer”等获取。)
The FAM21C protein, a member of the FAM21 family, is a critical component of the WASH (Wiskott-Aldrich syndrome protein and SCAR homologue) complex, which regulates actin nucleation and membrane trafficking in eukaryotic cells. FAM21C acts as a scaffolding protein, mediating interactions between the WASH complex and retromer machinery to facilitate endosomal sorting and cargo retrieval. It directs the recruitment of the complex to endosomal membranes, enabling the formation of actin patches that drive vesicle scission and retrograde transport. Dysregulation of FAM21C has been linked to defects in lysosomal function, autophagy, and cellular signaling, implicating its role in neurodegenerative disorders and cancer. Recombinant human FAM21C protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for structural and functional studies. Its purified form enables biochemical analyses, including binding assays, interaction mapping, and exploration of post-translational modifications. Research on recombinant FAM21C enhances understanding of intracellular trafficking pathways, disease mechanisms, and potential therapeutic targets.
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