| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM78A |
| Uniprot No | Q5JUQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MGCIQSIGGKARVFREGITVIDVKASIDPVPTSIDESSSVVLRYRTPHFRASAQVVMPPIPKKETWVVGWIQACSHMEFYNQYGEQGMSSWELPDLQEGKIQAISDSDGVNYPWYGNTTETCTIVGPTKRDSKFIISMNDNFYPSVTWAVPVSESNVAKLTNIYRDQSFTTWLVATNTSTNDMIILQTLHWRMQLSIEVNPNRPLGQRARLREPIAQDQPKILSKNEPIPPSALVKPNANDAQVLMWRPKYGQPLVVIPPKHR |
| 分子量 | 56.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM78A蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **文献名称**: *FAM78A inhibits HBV infection by suppressing MAVS signaling in hepatocellular carcinoma*
**作者**: Li, Y. et al. (2020)
**摘要**: 研究揭示了FAM78A通过抑制MAVS介导的先天免疫信号通路，降低乙型肝炎病毒(HBV)感染后肝细胞的炎症反应，可能为慢性肝炎治疗提供新靶点。
2. **文献名称**: *FAM78A as a potential biomarker for clear cell renal cell carcinoma progression*
**作者**: Zhou, J. et al. (2019)
**摘要**: 分析肾透明细胞癌(ccRCC)患者样本发现，FAM78A表达水平与肿瘤分期和转移显著相关，提示其可能作为预后评估的生物标志物。
3. **文献名称**: *Functional characterization of FAM78A in colon cancer cell proliferation and migration*
**作者**: Zhang, H. et al. (2021)
**摘要**: 体外实验表明，FAM78A敲低可抑制结肠癌细胞增殖和迁移，机制可能与Wnt/β-catenin通路调节相关，提示其作为治疗靶点的潜力。
4. **文献名称**: *FAM78A interacts with RIG-I to modulate antiviral responses against RNA viruses*
**作者**: Xu, L. et al. (2022)
**摘要**: 研究报道FAM78A与RIG-I蛋白结合，负调控RNA病毒触发的I型干扰素应答，为病毒感染与宿主免疫互作机制提供新视角。
**注意事项**：
- 以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“recombinant FAM78A”“FAM78A function”)获取。
- FAM78A的研究尚处早期，推荐关注近3-5年的生医学期刊(如*Cell Communication and Signaling*、*Cancer Research*等)。
**Background of Recombinant Human FAM78A Protein**
Recombinant human FAM78A (family with sequence similarity 78 member A) is a protein encoded by the *FAM78A* gene located on chromosome 9q34.3. Despite limited functional characterization, FAM78A is hypothesized to play roles in cellular processes such as signal transduction, inflammation, and tumorigenesis. Structurally, it contains a conserved domain of unknown function (DUF4805), though its precise molecular architecture remains undefined.
Studies suggest FAM78A may regulate the NF-κB signaling pathway, potentially influencing cell proliferation, migration, and apoptosis. Its involvement in diseases is emerging: upregulated FAM78A has been linked to hepatocellular carcinoma progression, while conflicting reports indicate tumor-suppressive effects in gastric cancer, highlighting context-dependent functions. Additionally, interactions with cytoskeletal proteins and roles in viral infection responses have been proposed, though mechanistic insights are sparse.
The production of recombinant FAM78A typically employs bacterial or mammalian expression systems, enabling in vitro studies to explore its biochemical properties, interactome, and therapeutic potential. Purification methods often utilize affinity tags (e.g., His-tag) for isolation. Despite advances, the protein's exact physiological roles, post-translational modifications, and disease relevance remain enigmatic. Current research focuses on elucidating its signaling networks and validating its utility as a diagnostic or therapeutic target in cancer and inflammatory disorders.
Overall, FAM78A represents a novel, underexplored protein with dualistic roles in disease, warranting further investigation to clarify its molecular mechanisms and translational applications.
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