| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM71F2 |
| Uniprot No | Q6NXP2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MSKIRGLPPEVREPGPGVELGVENGLLCQLIHSPEFNLFSNSVVFESNFIQTHVPEADFQVTKPGNWRDVCEGSATVILGVTSSVPSLPLPNVLLMANVTWPQGPFTTWSTPGVAPVINLSRLLPLKYVELRIYDRLQRILRVRTVTEKIYYLKLHEKHPEIVFQFWVRLVKILQKGLSITTKDPRIKFTHCLVPKMPTNSTETTPENSLLSSPQPSEPLVLLAAEQTSGSFSQLSGKPQLTADRNNDTAIEIDNCSSYKIPSPVASPINLNIPMRAALSHSLWEQEDWNEHLLQVHIASYLGEHFLGA |
| 分子量 | 60.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人FAM71F2蛋白的研究目前较为有限，以下是模拟的参考文献示例(请注意，部分内容可能基于合理推测，具体文献需通过学术数据库确认)：
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1. **标题**: *Expression and Functional Analysis of Recombinant Human FAM71F2 in Sperm Development*
**作者**: Li X, Zhang Y, et al.
**摘要**: 本研究首次在大肠杆菌系统中成功表达重组人FAM71F2蛋白，并通过质谱验证其正确性。体外实验表明，FAM71F2可能与精子运动相关蛋白(如CFAP家族)相互作用，推测其参与精子线粒体鞘的形成或功能调控。
2. **标题**: *Structural Characterization of FAM71F2 Using Cryo-EM and AlphaFold Prediction*
**作者**: Wang H, Chen L, et al.
**摘要**: 结合冷冻电镜与AlphaFold2预测，揭示了FAM71F2的三维结构特征。结果表明，该蛋白具有独特的α螺旋结构域，可能在细胞质-线粒体界面介导能量代谢相关信号通路，为功能研究提供结构基础。
3. **标题**: *FAM71F2 Knockout Mice Reveal Its Role in Male Fertility*
**作者**: Tanaka K, et al.
**摘要**: 通过构建FAM71F2基因敲除小鼠模型，发现雄性小鼠精子活力显著下降，线粒体膜电位异常。重组FAM71F2蛋白回补实验部分恢复了表型，支持其在精子能量代谢中的潜在作用。
4. **标题**: *Proteomic Screening of FAM71F2-Interacting Proteins in Testicular Tissue*
**作者**: Zhou M, Liu S, et al.
**摘要**: 利用Co-IP结合质谱技术，鉴定了FAM71F2在睾丸组织中的相互作用蛋白网络，包括TUBB4B、ATP5F1等。推测其通过调控线粒体ATP合成相关复合物，影响精子功能。
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**备注**：以上文献为模拟生成，实际研究中请通过PubMed或Web of Science等平台，以关键词“FAM71F2”、“recombinant protein”或“sperm mitochondria”进一步检索确认。
**Background of Recombinant Human FAM71F2 Protein**
The FAM71F2 (Family with Sequence Similarity 71 Member F2) gene encodes a protein predominantly expressed in human testicular tissues, suggesting a potential role in male reproductive biology. As a member of the FAM71 family, its exact physiological function remains under investigation, though studies imply associations with spermatogenesis and sperm function. The protein is predicted to contain conserved structural domains, including potential transmembrane regions, hinting at involvement in membrane-related processes or cell signaling.
Recombinant human FAM71F2 protein is artificially produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to explore interactions, post-translational modifications, and cellular localization. Preliminary evidence links FAM71F2 to sperm motility and structural integrity, with abnormal expression observed in certain cases of male infertility. Additionally, bioinformatics analyses suggest tissue-specific expression patterns, reinforcing its testis-enriched profile.
Current research focuses on elucidating its molecular mechanisms, including potential roles in protein-protein interactions, cytoskeletal organization, or enzymatic activities. The availability of recombinant FAM71F2 aids in antibody development, mechanistic studies, and exploring its diagnostic or therapeutic relevance in reproductive disorders. Further studies are needed to fully define its biological significance and clinical implications.
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