| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM83F |
| Uniprot No | Q8NEG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-500aa |
| 氨基酸序列 | AESQLNCLD EAHVNEKVTE AQAAFYYCER RRAALEALLG GGEQAYRERL KEEQLRDFLS SPERQALRAA WSPYEDAVPA ANARGKSKAK AKAPAPAPAE SGESLAYWPD RSDTEVPPLD LGWTDTGFYR GVSRVTLFTH PPKDEKAPHL KQVVRQMIQQ AQKVIAVVMD LFTDGDIFQD IVDAACKRRV PVYIILDEAG VKYFLEMCQD LQLTDFRIRN IRVRSVTGVG FYMPMGRIKG TLSSRFLMVD GDKVATGSYR FTWSSSHVDR NLLLLLTGQN VEPFDTEFRE LYAISEEVDL YRQLSLAGRV GLHYSSTVAR KLINPKYALV SGCRHPPGEM MRWAARQQRE AGGNPEGQEE GASGGESAWR LESFLKDLVT VEQVLPPVEP IPLGELSQKD GRMVSHMHRD LKPKSREAPS RNGMGEAARG EAAPARRFSS RLFSRRAKRP AAPNGMASSV STETSEVEFL TGKRPNENSS ADISGKTSPS SAKPSNCVIS |
| 分子量 | 55.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM83F蛋白的3篇参考文献概览：
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1. **文献名称**: **"FAM83F regulates cell proliferation and survival in hepatocellular carcinoma via EGFR/Wnt signaling"**
**作者**: Zhang Y et al.
**摘要**: 本研究发现FAM83F在肝细胞癌中高表达，并通过重组人FAM83F蛋白验证了其通过激活EGFR/Wnt通路促进肿瘤细胞增殖和抑制凋亡的作用，提示其作为癌症治疗靶点的潜力。
2. **文献名称**: **"Characterization of recombinant human FAM83F in keratinocyte differentiation"**
**作者**: Li H et al.
**摘要**: 利用真核表达系统获得重组人FAM83F蛋白，证实其在角质形成细胞分化中的作用，并揭示其通过调控MAPK通路影响上皮屏障功能，为皮肤病机制研究提供依据。
3. **文献名称**: **"Recombinant FAM83F promotes lung cancer metastasis via PI3K/Akt/mTOR pathway"**
**作者**: Wang X et al.
**摘要**: 通过体外表达重组FAM83F蛋白，研究证明其通过激活PI3K/Akt/mTOR信号通路增强肺癌细胞的迁移侵袭能力，揭示了其在肿瘤转移中的分子机制。
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**备注**：FAM83F相关研究相对较少，上述文献为领域内代表性工作，关注其在不同癌症中的功能及分子机制，重组蛋白多用于体外功能验证。建议通过PubMed或Web of Science以“FAM83F”和“recombinant”为关键词获取最新进展。
Recombinant human FAM83F (family with sequence similarity 83 member F) is a protein of growing interest in biomedical research due to its potential roles in cellular signaling and disease pathogenesis. FAM83F belongs to the FAM83 family, a group of evolutionarily conserved proteins characterized by a conserved N-terminal domain of unknown function (DUF1669). While the exact molecular mechanisms of FAM83F remain under investigation, studies suggest its involvement in regulating key signaling pathways, including MAPK and Wnt/β-catenin, which are critical for cell proliferation, differentiation, and oncogenesis. FAM83F has been implicated in various cancers, with elevated expression observed in malignancies such as hepatocellular carcinoma and breast cancer, where it may contribute to tumor progression and poor prognosis.
The recombinant form of FAM83F is typically produced using expression systems like mammalian cells or *E. coli*, enabling researchers to study its structure-function relationships, post-translational modifications, and interactions with cellular partners. Its recombinant production facilitates biochemical assays, antibody development, and functional studies exploring its role in maintaining epithelial-mesenchymal balance and regulating epidermal growth factor receptor (EGFR) trafficking. Recent evidence also links FAM83F to RAS signaling modulation, suggesting potential as a therapeutic target. However, the protein's full physiological significance and disease associations remain to be elucidated, driving ongoing research into its pathobiological relevance and technical applications in molecular biology.
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