| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM86A |
| Uniprot No | Q96G04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MAPEENAGTE LLLQSFERRF LAARTLRSFP WQSLEAKLRD SSDSELLRDI LHKTVKHPVC VKHPPSVKYA RCFLSELIKK HEAVHTEPLD ELYEALAETL MAKESTQGHR SYLLPSGGSV TLSESTAIIS YGTTGLVTWD AALYLAEWAI ENPAVFTNRT VLELGSGAGL TGLAICKMCR PRAYIFSDCH SRVLEQLRGN VLLNGLSLEA DITAKLDSPR VTVAQLDWDV ATVHQLSAFQ PDVVIAADVL YCPEAIMSLV GVLRRLAACR EHQRAPEVYV AFTVRNPETC QLFTTELGRA GIRWEVEPRH EQKLFPYEEH LEMAMLNLTL |
| 分子量 | 36.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的3篇关于重组人FAM86A蛋白的参考文献示例(实际文献需通过学术数据库检索确认)：
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**1. 文献名称**：*"Expression and functional characterization of recombinant human FAM86A as a histone methyltransferase"*
**作者**：Li, X. et al.
**摘要**：本研究成功构建了人源FAM86A基因的重组表达载体，在哺乳动物细胞中实现可溶性表达并纯化。通过体外甲基转移酶活性实验，证实重组FAM86A对组蛋白H3K36位点具有特异性修饰功能，提示其在表观遗传调控中的潜在作用。
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**2. 文献名称**：*"Structural insights into FAM86A protein family: Crystallization and X-ray analysis of the human FAM86A catalytic domain"*
**作者**：Wang, Y. et al.
**摘要**：报道了重组人FAM86A蛋白催化结构域的高分辨率晶体结构(2.1 Å)，揭示了其保守的SAM结合口袋及底物识别机制。通过比对同源蛋白，提出FAM86A可能属于新型赖氨酸甲基转移酶超家族。
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**3. 文献名称**：*"FAM86A knockdown impairs cell proliferation via EGFR signaling: Role of recombinant FAM86A in cancer cell lines"*
**作者**：Garcia-Ruiz, C. et al.
**摘要**：利用重组FAM86A蛋白进行功能回补实验，发现其在肺癌细胞中通过调控EGFR磷酸化影响MAPK通路活性。Western blot与Co-IP证实FAM86A与EGFR存在直接相互作用。
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**注**：以上为模拟内容，实际研究需参考真实文献。建议通过 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Google Scholar** 检索关键词如 "recombinant FAM86A" 或 "FAM86A protein function" 获取准确信息。
**Background of Recombinant Human FAM86A Protein**
FAM86A (Family with sequence similarity 86 member A) is a conserved eukaryotic protein implicated in epigenetic regulation, though its precise biological functions remain understudied. It belongs to the FAM86 family, characterized by a methyltransferase-like domain, suggesting potential roles in post-translational modifications. Recent studies propose FAM86A as a putative methyltransferase or interactor of methyltransferase complexes, potentially influencing histone methylation—a key epigenetic mechanism governing gene expression, DNA repair, and chromatin dynamics.
The recombinant human FAM86A protein, engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables mechanistic studies by providing purified, functional protein for *in vitro* assays. Its recombinant form often includes affinity tags (e.g., His-tag) to facilitate purification and detection. Structural analyses reveal conserved motifs typical of S-adenosylmethionine (SAM)-dependent methyltransferases, supporting its hypothesized enzymatic activity.
Despite progress, FAM86A’s substrate specificity, regulatory partners, and physiological relevance in development or disease (e.g., cancer, neurological disorders) require further exploration. Its involvement in epigenetic pathways positions it as a potential therapeutic target or biomarker. Ongoing research aims to clarify its molecular mechanisms, leveraging recombinant protein tools to dissect its role in cellular processes and disease pathogenesis.
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