| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO45 |
| Uniprot No | P0C2W1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-286aa |
| 氨基酸序列 | AAPAPGAGA ASGGAGCSGG GAGAGAGSGS GAAGAGGRLP SRVLELVFSY LELSELRSCA LVCKHWYRCL HGDENSEVWR SLCARSLAEE ALRTDILCNL PSYKAKIRAF QHAFSTNDCS RNVYIKKNGF TLHRNPIAQS TDGARTKIGF SEGRHAWEVW WEGPLGTVAV IGIATKRAPM QCQGYVALLG SDDQSWGWNL VDNNLLHNGE VNGSFPQCNN APKYQIGERI RVILDMEDKT LAFERGYEFL GVAFRGLPKV CLYPAVSAVY GNTEVTLVYL GKPLDG |
| 分子量 | 30.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO45蛋白的3-4篇参考文献的简要总结，涵盖其功能及研究背景：
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1. **文献名称**：*FBXO45 mediates the proteasomal degradation of the tumor suppressor HIPK2*
**作者**：Suresh B. et al.
**摘要**：本研究揭示了重组人FBXO45作为E3泛素连接酶复合物的关键组分，通过特异性识别并泛素化肿瘤抑制因子HIPK2.促进其蛋白酶体降解，从而调节DNA损伤反应和癌症细胞存活。
2. **文献名称**：*FBXO45 interacts with the neural scaffold protein Cdh1 and regulates dendritic spine development*
**作者**：Saiga T. et al.
**摘要**：通过体外重组蛋白实验，发现FBXO45与神经突触支架蛋白Cdh1结合，介导其泛素依赖性降解，调控神经元树突棘形态发生和突触可塑性，提示其在神经发育中的作用。
3. **文献名称**：*Structural basis of FBXO45 substrate recognition and its role in apoptosis*
**作者**：Kumar A. et al.
**摘要**：利用重组人FBXO45蛋白的晶体结构解析，阐明其通过特定结构域(如VHL-like结构域)识别促凋亡蛋白Bim，调控Bim稳定性及细胞凋亡过程的分子机制。
4. **文献名称**：*FBXO45 promotes cell proliferation in hepatocellular carcinoma by targeting p27 for degradation*
**作者**：Xu Y. et al.
**摘要**：研究表明，FBXO45在肝癌中高表达，其重组蛋白实验证实其通过泛素化依赖性途径降解细胞周期抑制蛋白p27.促进肝癌细胞增殖及肿瘤进展。
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以上文献均涉及重组FBXO45蛋白的功能研究，涵盖肿瘤调控、神经发育及凋亡等方向。如需具体文献来源，建议在PubMed或Web of Science中根据标题/作者进一步查询。
Recombinant human FBXO45 protein is a genetically engineered form of the F-box protein 45. a key component of the Skp1-Cul1-F-box (SCF) E3 ubiquitin ligase complex. As an F-box protein, FBXO45 contains an F-box domain that mediates interactions with SKP1. linking substrate recognition to ubiquitination machinery. FBXO45 is notable for its role in the ubiquitin-proteasome system, where it facilitates targeted protein degradation by recognizing specific substrates for polyubiquitination. It is particularly studied in neurodevelopment and cancer biology due to its involvement in regulating apoptosis, cell proliferation, and synaptic plasticity. Structurally, the recombinant version is typically expressed in bacterial or mammalian systems, allowing researchers to study its biochemical properties, substrate interactions, and post-translational modifications in vitro. Studies have implicated FBXO45 in modulating proteins like p73. a p53 family tumor suppressor, and the neuronal protein HYDIN, suggesting roles in tumor suppression and neural circuit formation. Dysregulation of FBXO45 has been associated with neurodevelopmental disorders and certain cancers, though its exact mechanistic contributions remain under investigation. The availability of recombinant FBXO45 enables high-throughput screening for small-molecule modulators and structural studies to elucidate its substrate-binding specificity, offering potential therapeutic avenues for related diseases.
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