| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO47 |
| Uniprot No | Q5MNV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452aa |
| 氨基酸序列 | MASRINTNFT LIPNQKLRRS NRQTSCYSKT LGSGFQPIST FGNFKALPLE IFQIILKYLS VKDISMLSMV SKTVSQHIIN YISTSSGSKR LLLQDFHNLE LPDRRQDSAI LEHYRSLGLL FKRCTLLLPT KERLKYIHKI LTEVSCFKFN GCAAPMQCLG LTCYGMFLQT LTAGWDELEC HRVYNFLCEL TNLCRKIQMA VCSKPGSAQK LELRIRLFCR NVLLDHWTHR SDSAFWLTRI LKPWPMVNQA RLLYIIFGPI SPQDGQVVWQ EMIEEPTDEF SLKGLADAIK LLYDASTKEW TADDVISLVD ELSVVPREWL LENNARLLML SGNNICFSFM ASKAVNGRTI ELARLVVFLA LVCEKELYCM DWTVKMMQKV CKVFSTPVER KNFLQNVANA FACVIMEMLQ SIMSGDRDED DRSFLNLFHL VHAQANFHKE VLYLTMNTPL ST |
| 分子量 | 51.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO47蛋白的示例性参考文献(内容为假设性概括，仅供参考)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human FBXO47 in E. coli*
**作者**：Zhang L, Wang Y, Chen J.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人FBXO47蛋白，验证了其与Skp1蛋白的结合能力，表明其可能参与SCF泛素连接酶复合体的组装，为后续功能研究奠定基础。
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2. **文献名称**：*FBXO47 Regulates CDT1 Ubiquitination During DNA Replication*
**作者**：Smith R, Tanaka K, Lee H.
**摘要**：文章发现重组人FBXO47在体外泛素化实验中特异性结合并降解CDT1蛋白，调控细胞S期进程，揭示了其在DNA复制许可中的潜在作用机制。
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3. **文献名称**：*Structural Insights into FBXO47 Substrate Recognition via Cryo-EM*
**作者**：Johnson M, Liu X, White AP.
**摘要**：利用冷冻电镜解析了重组人FBXO47蛋白的三维结构，阐明其F-box结构域和C端底物识别区的构象变化，为靶向FBXO47的药物设计提供依据。
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4. **文献名称**：*FBXO47 Overexpression Promotes Tumor Growth in Colorectal Cancer Models*
**作者**：Gupta S, Park S, Kim D.
**摘要**：通过重组人FBXO47蛋白的功能实验，证明其在结直肠癌细胞中异常高表达，并通过泛素化降解抑癌蛋白驱动肿瘤进展，提示其作为潜在治疗靶点。
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**注**：以上文献为模拟内容，实际研究中需通过数据库(如PubMed、Web of Science)验证真实性。如需具体文献，建议结合研究关键词进一步检索。
Recombinant human FBXO47 protein is a genetically engineered form of the F-box protein 47. a member of the F-box family characterized by the presence of a conserved F-box domain. These proteins function as substrate-recognition components in Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complexes, which mediate targeted protein ubiquitination and subsequent degradation via the proteasome. FBXO47 plays roles in diverse cellular processes, including cell cycle regulation, DNA repair, and meiosis. Studies suggest its involvement in maintaining genomic stability during spermatogenesis by regulating chromosome pairing and crossover formation. Dysregulation of FBXO47 has been implicated in cancers, where it may act as an oncogene or tumor suppressor depending on context. For instance, it reportedly stabilizes oncogenic proteins in some malignancies but promotes apoptosis in others. The recombinant form is typically produced in bacterial or mammalian expression systems, enabling in vitro studies of its biochemical interactions, structural features, and substrate-binding specificity. Its purification facilitates investigations into post-translational modifications, dimerization properties, and enzymatic activity within SCF complexes. Current research focuses on identifying its physiological substrates, delineating regulatory mechanisms, and exploring therapeutic potential through small-molecule modulators. The protein's conserved F-box domain and C-terminal protein-binding motifs make it a valuable tool for probing ubiquitination pathways and disease-associated mutations.
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