| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO7 |
| Uniprot No | Q9Y3I1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522aa |
| 氨基酸序列 | MRLRVRLLKRTWPLEVPETEPTLGHLRSHLRQSLLCTWGYSSNTRFTITLNYKDPLTGDEETLASYGIVSGDLICLILQDDIPAPNIPSSTDSEHSSLQNNEQPSLATSSNQTSIQDEQPSDSFQGQAAQSGVWNDDSMLGPSQNFEAESIQDNAHMAEGTGFYPSEPMLCSESVEGQVPHSLETLYQSADCSDANDALIVLIHLLMLESGYIPQGTEAKALSMPEKWKLSGVYKLQYMHPLCEGSSATLTCVPLGNLIVVNATLKINNEIRSVKRLQLLPESFICKEKLGENVANIYKDLQKLSRLFKDQLVYPLLAFTRQALNLPDVFGLVVLPLELKLRIFRLLDVRSVLSLSAVCRDLFTASNDPLLWRFLYLRDFRDNTVRVQDTDWKELYRKRHIQRKESPKGRFVMLLPSSTHTIPFYPNPLHPRPFPSSRLPPGIIGGEYDQRPTLPYVGDPISSLIPGPGETPSQFPPLRPRFDPVGPLPGPNPILPGRGGPNDRFPFRPSRGRPTDGRLSFM |
| 分子量 | 84.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人FBXO7蛋白相关的参考文献示例(部分基于真实研究，内容已简化概括)：
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1. **"FBXO7 mutations in Parkinson's disease and multiple system atrophy"**
*作者：Di Fonzo A, et al. (2009)*
**摘要**：该研究首次将FBXO7基因突变与早发性帕金森病(PD)联系起来，发现FBXO7通过调控线粒体自噬和泛素-蛋白酶体系统参与神经元存活，突变导致其功能丧失，从而影响异常蛋白的清除。
2. **"Structural insights into the interaction of FBXO7 with S-Phase Kinase-Associated Protein 1 (Skp1)"**
*作者：Zhao J, et al. (2018)*
**摘要**：本文解析了重组人FBXO7蛋白与Skp1的复合物晶体结构，揭示了FBXO7通过N端结构域特异性结合Skp1的分子机制，为阐明SCF泛素连接酶复合物的组装及底物识别提供了结构基础。
3. **"FBXO7 regulates mitophagy through Parkin-dependent pathways"**
*作者：Burchell VS, et al. (2013)*
**摘要**：研究证明FBXO7与Parkin蛋白协同调控线粒体质量，重组人FBXO7的表达可挽救帕金森病模型中的线粒体功能障碍，强调了其在维持线粒体稳态中的关键作用。
4. **"Recombinant production and functional characterization of human FBXO7 in E. coli"**
*作者：Liu Y, et al. (2020)*
**摘要**：报道了利用大肠杆菌表达系统高效制备重组人FBXO7蛋白的方法，并通过体外泛素化实验验证了其与Skp1/Cullin1复合物的结合能力及泛素连接酶活性。
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*注：以上文献标题及作者为示例性质，实际引用时请以正式出版物为准。若需真实文献，建议通过PubMed或Google Scholar检索关键词“FBXO7 recombinant”或“FBXO7 Parkinson”。*
Recombinant human FBXO7 protein is a key component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex, belonging to the F-box protein family. FBXO7 contains an F-box domain that facilitates interaction with Skp1. linking it to the core SCF complex, and additional domains like the proline-rich region (PRR) and ubiquitin-like (UBX) domain, which mediate substrate recognition and protein interactions. Functionally, FBXO7 plays critical roles in proteasome-mediated protein degradation, cell cycle regulation, mitochondrial quality control (via mitophagy), and immune signaling. Dysregulation or mutations in FBXO7 are linked to neurodegenerative disorders, particularly early-onset Parkinson’s disease (PARK15), where impaired mitophagy and protein homeostasis contribute to pathogenesis. Recombinant FBXO7 is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. It serves as a vital tool for studying enzymatic activity, protein-protein interactions, and disease mechanisms. Research on FBXO7 aids in exploring therapeutic strategies for Parkinson’s and other disorders linked to ubiquitin-proteasome dysfunction.
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