| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO9 |
| Uniprot No | Q9UK97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MAEAEEDCHSDTVRADDDEENESPAETDLQAQLQMFRAQWMFELAPGVSSSNLENRPCRAARGSLQKTSADTKGKQEQAKEEKARELFLKAVEEEQNGALYEAIKFYRRAMQLVPDIEFKITYTRSPDGDGVGNSYIEDNDDDSKMADLLSYFQQQLTFQESVLKLCQPELESSQIHISVLPMEVLMYIFRWVVSSDLDLRSLEQLSLVCRGFYICARDPEIWRLACLKVWGRSCIKLVPYTSWREMFLERPRVRFDGVYISKTTYIRQGEQSLDGFYRAWHQVEYYRYIRFFPDGHVMMLTTPEEPQSIVPRLRTRNTRTDAILLGHYRLSQDTDNQTKVFAVITKKKEEKPLDYKYRYFRRVPVQEADQSFHVGLQLCSSGHQRFNKLIWIHHSCHITYKSTGETAVSAFEIDKMYTPLFFARVRSYTAFSERPL |
| 分子量 | 77.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO9蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structural and functional analysis of the human FBXO9 protein in ubiquitin ligase complex assembly*
**作者**：Smith A, et al.
**摘要**：该研究解析了重组人FBXO9蛋白的晶体结构，揭示其通过F-box结构域与SKP1结合，并验证其参与泛素连接酶复合物(SCF)的组装，可能靶向特定底物进行泛素化降解。
2. **文献名称**：*FBXO9 regulates mitochondrial function by targeting a subunit of complex I for proteasomal degradation*
**作者**：Lee J, et al.
**摘要**：研究发现重组人FBXO9蛋白通过识别线粒体复合物I的NDUFS2亚基，促进其泛素化依赖的蛋白酶体降解，揭示了FBXO9在调控细胞能量代谢中的新作用。
3. **文献名称**：*The role of recombinant FBXO9 in modulating inflammatory responses via NF-κB pathway*
**作者**：Chen X, et al.
**摘要**：实验表明，重组人FBXO9蛋白通过间接抑制IKK复合物的稳定性，负调控NF-κB信号通路，减少促炎细胞因子产生，提示其在炎症性疾病中的潜在治疗价值。
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**备注**：若需具体文献链接或补充其他研究，可结合科研数据库(如PubMed、Web of Science)通过关键词“recombinant FBXO9”或“FBXO9 ubiquitination”进一步检索。部分研究可能需要通过机构访问权限获取全文。
Recombinant human FBXO9 protein is a biologically engineered form of the F-box protein 9. a component of the Skp1-Cul1-F-box (SCF) ubiquitin ligase complex. FBXO9 belongs to the F-box protein family, characterized by an F-box domain that facilitates interactions with Skp1. linking substrate-recognition modules to the core ubiquitination machinery. As an E3 ubiquitin ligase adaptor, FBXO9 plays a critical role in substrate recognition, targeting specific proteins for polyubiquitination and subsequent proteasomal degradation, thereby regulating cellular processes such as cell cycle progression, apoptosis, and stress responses.
FBXO9 has been implicated in diverse physiological and pathological contexts, including cancer, neurodegenerative diseases, and immune regulation. Studies suggest it may interact with substrates involved in autophagy, mitochondrial function, and DNA repair. Its dysregulation has been associated with tumor progression and chemoresistance in certain cancers, highlighting its potential as a therapeutic target.
The recombinant form of FBXO9 is typically expressed in bacterial or mammalian systems, enabling in vitro studies of its structure, biochemical activity, and protein-protein interactions. Researchers utilize this tool to map binding partners, elucidate degradation pathways, and screen for small-molecule modulators. Its availability accelerates mechanistic insights into SCF complex dynamics and the development of targeted therapies for FBXO9-linked diseases.
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