| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXW11 |
| Uniprot No | Q9UKB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542aa |
| 氨基酸序列 | MEPDSVIEDKTIELMCSVPRSLWLGCANLVESMCALSCLQSMPSVRCLQISNGTSSVIVSRKRPSEGNYQKEKDLCIKYFDQWSESDQVEFVEHLISRMCHYQHGHINSYLKPMLQRDFITALPEQGLDHIAENILSYLDARSLCAAELVCKEWQRVISEGMLWKKLIERMVRTDPLWKGLSERRGWDQYLFKNRPTDGPPNSFYRSLYPKIIQDIETIESNWRCGRHNLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR |
| 分子量 | 69.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into the recognition of β-TrCP by FBXW11 in the Wnt/β-catenin signaling pathway"**
- *Author: Kudo Y, et al.*
- 摘要：研究解析了FBXW11与β-TrCP相互作用的结构机制，阐明其通过泛素-蛋白酶体系统调控β-catenin降解，影响Wnt信号通路及肿瘤发生。
2. **"FBXW11/hCdc4 targets p53 for degradation and promotes cancer cell survival"**
- *Author: Dias SS, et al.*
- 摘要：发现FBXW11作为E3泛素连接酶复合物组分，通过泛素化促肿瘤抑制因子p53降解，揭示其在胃癌细胞增殖和化疗耐药中的功能。
3. **"Recombinant human FBXW11 protein mediates substrate ubiquitylation in neurodegenerative disease models"**
- *Author: Smith J, et al.*
- 摘要：报道重组人FBXW11蛋白的体外表达及活性验证，证实其在帕金森病模型中促进α-synuclein异常聚集蛋白的泛素化清除。
4. **"FBXW11 regulates pluripotency by controlling OCT4 protein stability in embryonic stem cells"**
- *Author: Matsumoto A, et al.*
- 摘要：研究利用重组FBXW11蛋白证明其通过动态调控OCT4泛素化水平，影响胚胎干细胞自我更新与分化过程。
(注：以上文献信息为示例，实际引用需以真实出版物为准。)
**Background of Recombinant Human FBXW11 Protein**
Recombinant human FBXW11 (F-box/WD repeat-containing protein 11), also known as β-TrCP2 or HOS, is a member of the F-box protein family that plays a critical role in the ubiquitin-proteasome system. As a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex, FBXW11 mediates the targeted degradation of specific proteins through phosphorylation-dependent interactions. It contains an F-box domain for binding SKP1 and WD40 repeats for substrate recognition, enabling regulation of diverse cellular processes, including cell cycle progression, DNA damage response, and signal transduction pathways such as Wnt/β-catenin and NF-κB.
FBXW11 targets key regulatory proteins like β-catenin, IκB, and CDC25A for proteasomal degradation, impacting oncogenesis, immune responses, and neurodevelopment. Dysregulation of FBXW11 has been linked to cancers, neurodegenerative disorders, and developmental defects. Recombinant FBXW11 protein, produced via expression systems like *E. coli* or baculovirus-infected insect cells, is widely used in structural studies, biochemical assays, and drug discovery to explore its interactions, substrate specificity, and therapeutic potential. Its availability in purified form facilitates research into molecular mechanisms underlying diseases and the development of targeted therapies modulating ubiquitination pathways.
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