| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FCHO2 |
| Uniprot No | Q0JRZ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-810aa |
| 氨基酸序列 | MVMAYFVENFWGEKNSGFDVLYHNMKHGQISTKELADFVRERATIEEAYSRSMTKLAKSASNYSQLGTFAPVWDVFKTSTEKLANCHLDLVRKLQELIKEVQKYGEEQVKSHKKTKEEVAGTLEAVQTIQSITQALQKSKENYNAKCVEQERLKKEGATQREIEKAAVKSKKATDTYKLYVEKYALAKADFEQKMTETAQKFQDIEETHLIHIKEIIGSLSNAIKEIHLQIGQVHEEFINNMANTTVESLIQKFAESKGTGKERPGLIEFEECDTASAVEGIKPRKRKTFALPGIIKKEKDAESVECPDADSLNIPDVDEEGYSIKPETNQNDTKENHFYSSSDSDSEDEEPKKYRIEIKPMHPNNSHHTMASLDELKVSIGNITLSPAISRHSPVQMNRNLSNEELTKSKPSAPPNEKGTSDLLAWDPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKLSGINEIPRPFSPPVTSNTSPPPAAPLARAESSSSISSSASLSAANTPTVGVSRGPSPVSLGNQDTLPVAVALTESVNAYFKGADPTKCIVKITGDMTMSFPSGIIKVFTSNPTPAVLCFRVKNISRLEQILPNAQLVFSDPSQCDSNTKDFWMNMQAVTVYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSSISEKSENGGSGSLRAKFDLSEGPSKPTTLAVQFLSEGSTLSGVDFELVGTGYRLSLIKKRFATGRYLADC |
| 分子量 | 88.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FCHO2蛋白的3条参考文献及其摘要概括：
1. **文献名称**: *FCHo proteins are nucleators of clathrin-coated pits*
**作者**: Henne WM, Boucrot E, Meinecke M, et al.
**摘要**: 该研究揭示了FCHo1和FCHo2(包括重组人FCHO2蛋白)作为网格蛋白包被小窝(clathrin-coated pit)形成的起始因子，通过其F-BAR结构域结合膜脂并招募下游接头蛋白AP2.调控内吞过程。
2. **文献名称**: *Structural basis of FCHo2 binding to the plasma membrane*
**作者**: Umasankar PK, Ma L, Thieman JR, et al.
**摘要**: 文章通过晶体结构和生化实验，解析了重组人FCHO2蛋白的F-BAR结构域与细胞膜相互作用的分子机制，表明其通过弯曲膜结构促进内吞囊泡的成型。
3. **文献名称**: *Functional characterization of FCHO2 phosphorylation in receptor endocytosis*
**作者**: Chen Z, Li J, Xu Y, et al.
**摘要**: 该研究利用重组人FCHO2蛋白突变体，发现其特定酪氨酸磷酸化位点对EGFR受体内吞至关重要，揭示了磷酸化修饰在调节FCHO2功能及信号转导中的作用。
注：以上文献为模拟示例，实际引用需根据具体研究内容检索PubMed或Web of Science获取准确信息。
Recombinant human FCHO2 (FCH domain-only protein 2) is a protein engineered for studies on clathrin-mediated endocytosis (CME), a critical cellular process for nutrient uptake, signal transduction, and membrane remodeling. FCHO2 belongs to the F-BAR protein family, known for sensing and inducing membrane curvature. It acts as an early-stage scaffold in CME, facilitating the assembly of clathrin-coated pits by recruiting adaptor proteins like EPS15 and intersectin. Structurally, FCHO2 contains an F-BAR domain for membrane binding and a μ-homology domain (μHD) that interacts with endocytic components.
The recombinant form of FCHO2 is typically produced using bacterial or mammalian expression systems, enabling biochemical and structural analyses. Researchers use it to dissect its role in initiating endocytosis, particularly how its membrane-deforming activity coordinates with downstream partners. Dysregulation of FCHO2 is linked to disorders involving defective endocytosis, including cancers, neurological diseases, and viral entry mechanisms (e.g., HIV, influenza).
Studies on recombinant FCHO2 also aim to identify therapeutic targets for modulating endocytic pathways. Its applications extend to drug screening platforms and understanding pathogenic exploitation of CME. Ongoing research focuses on resolving FCHO2’s 3D structure and regulatory networks, which remain incompletely characterized.
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