| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXW12 |
| Uniprot No | Q6X9E4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MSPTHQIQDPKHWNRIADSDYLWRSLSLQRWDCSNFTNQHLGTHTWKQFFLHQRRKELRLALAQPHNFIYKVTKNIAFETELAYLSGNRLTVDEQEKSIICSVSPKQELCAWDVQEGTMIWSSPVQEFHFSNLVTLPQMHLAITMDRKKTIKVWNCQDRDALAVLPMPQPCYCMEAYLTKDGPFLMVGDAAGDIYTFTLPGLRDVSKVTAFQYGIVLLHCSPDKKWVFACGTYSRTLPQVFLTESLLRPSEGSDPLSTLLPHKLCASACWTPKVKNRITLMSQSSTGKKTEFITFDLTTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGASDGYMIVFTSGPYLLLFSITGFLLQRFEDHQAAINNFWVDPCYVLTTSENSVHVYMWEEGGRHPYLRSCCHLENTWHDHTTDSCISSVMCDNASIVLRVRKVSDSSILVMYSLNT |
| 分子量 | 77.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBWX12蛋白的3条文献示例(注：FBXW12研究较少，部分内容为模拟概括，实际文献可能需要通过专业数据库验证)：
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1. **文献名称**: *F-box protein FBXW12 interacts with GRP78 to regulate mitochondrial function in HeLa cells*
**作者**: Chen L, et al.
**摘要**: 本研究报道了重组人FBXW12蛋白通过与GRP78结合调控线粒体能量代谢的机制。通过体外泛素化实验证明其可能作为SCF复合物底物识别组分参与蛋白质降解。
2. **文献名称**: *Structural characterization of recombinant human FBXW12 and its role in cancer cell apoptosis*
**作者**: Wang Y, et al.
**摘要**: 通过大肠杆菌系统表达了重组人FBXW12蛋白并解析其晶体结构，发现该蛋白在肺癌细胞中过表达可抑制PI3K/AKT通路，诱导caspase依赖性凋亡。
3. **文献名称**: *Identification of FBXW12 as a novel negative regulator of Wnt/β-catenin signaling pathway*
**作者**: Kim S, et al.
**摘要**: 利用重组FBXW12蛋白进行功能筛选，发现其通过促进β-catenin泛素化降解抑制结直肠癌细胞增殖，为潜在肿瘤治疗靶点提供依据。
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**注意**：FBXW12属于研究较少的F-box蛋白，上述文献为示例性质，实际研究中建议通过PubMed、Google Scholar等平台以**"FBXW12 recombinant"**或**"FBXW12 function"**为关键词检索最新文献。若研究有限，可扩展至FBXW家族其他成员(如FBXW7)的机制类比分析。
**Background of Recombinant Human FBXW12 Protein**
FBXW12 (F-box and WD repeat domain-containing 12) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This complex plays a crucial role in the ubiquitin-proteasome system, targeting specific proteins for proteasomal degradation to regulate cellular processes like cell cycle progression, signal transduction, and apoptosis. The FBXW12 protein contains characteristic F-box and WD40 repeat domains; the F-box facilitates interaction with SKP1. while WD40 repeats mediate substrate binding.
Despite its structural similarity to well-studied F-box proteins (e.g., FBXW7), FBXW12 remains poorly characterized. Limited studies suggest potential roles in neurological functions and cancer, though its precise physiological substrates and regulatory mechanisms are largely unknown. Its tissue-specific expression patterns, particularly in the brain and testis, hint at specialized biological roles.
Recombinant FBXW12 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies, including substrate identification, enzyme activity assays, and structural analysis. Its recombinant form is also valuable for exploring interactions within the SCF complex and screening potential modulators for therapeutic applications. Current research aims to unravel its biological significance, particularly in disease contexts, leveraging recombinant tools to bridge knowledge gaps in ubiquitin-mediated proteostasis.
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