| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXW9 |
| Uniprot No | Q5XUX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-488aa |
| 氨基酸序列 | MELPLGRCDD SRTWDDDSDP ESETDPDAQA KAYVARVLSP PKSGLAFSRP SQLSTPAASP SASEPRAASR VSAVSEPGLL SLPPELLLEI CSYLDARLVL HVLSRVCHAL RDLVSDHVTW RLRALRRVRA PYPVVEEKNF DWPAACIALE QHLSRWAEDG RWVEYFCLAE GHVASVDSVL LLQGGSLCLS GSRDRNVNLW DLRQLGTESN QVLIKTLGTK RNSTHEGWVW SLAAQDHRVC SGSWDSTVKL WDMAADGQQF GEIKASSAVL CLSYLPDILV TGTYDKKVTI YDPRDRMETR DALMGWGGPS RGSDIPPHRP ITHEAGPALL KHQQLHSRPV LTLLADDRHI ISGSEDHTLV VVDRRANSVL QRLQLDSYLL CMSYQEPQLW AGDNQGLLHV FANRNGCFQL IRSFDVGHSF PITGIQYSVG ALYTTSTDKT IRVHVPTDPP RTICTRRHDN GLNRVCAEGN LVVAGSGDLS LEVWRLQA |
| 分子量 | 50.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前关于重组人FBXW9蛋白的公开研究较为有限，以下内容基于相关领域知识的推测性示例，并非真实存在的文献。建议通过学术数据库(如PubMed、Google Scholar)查询最新进展：
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1. **文献名称**：*"Structural and Functional Analysis of Human FBXW9 in Ubiquitin-Mediated Proteolysis"*
**作者**：Chen L, et al.
**摘要**：解析了重组人FBXW9蛋白的晶体结构，表明其WD40结构域特异性识别降解底物，提示其在细胞周期调控中的作用。
2. **文献名称**：*"FBXW9 Regulates mTOR Signaling via Interaction with Deptor"*
**作者**：Kim H, et al.
**摘要**：研究发现FBXW9通过泛素化降解Deptor蛋白，激活mTOR通路，可能影响肿瘤细胞代谢和生长。
3. **文献名称**：*"The Role of FBXW9 in Neuronal Development: Insights from Knockout Models"*
**作者**：Wang Y, et al.
**摘要**：利用基因敲除技术，发现FBXW9缺失导致神经元分化异常，推测其通过调控Notch信号通路发挥作用。
4. **文献名称**：*"Emerging Roles of F-Box Proteins: Focus on FBXW9 in Immune Regulation"*
**作者**：Gupta R, et al.
**摘要**：综述中提及FBXW9可能通过介导免疫相关因子的降解，参与炎症反应的负向调控。
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**注意**：以上文献为假设性示例，反映F-box蛋白家族的典型研究方向。实际研究中FBXW9的具体功能仍需实验验证。建议结合“SCF泛素连接酶”“FBXW家族”等关键词进一步检索。
Recombinant human FBXW9 protein is a genetically engineered form of the F-box/WD repeat-containing protein 9. a member of the F-box protein family. These proteins are critical components of the ubiquitin-proteasome system, functioning as substrate-recognition modules in Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complexes. FBXW9. characterized by its F-box domain and WD40 repeats, is hypothesized to mediate targeted protein degradation by binding specific phosphodegrons on substrate proteins, tagging them for proteasomal destruction. However, its precise biological roles remain less defined compared to well-studied homologs like FBXW7.
The recombinant version is typically produced in bacterial or mammalian expression systems, enabling studies on its structure, interactions, and enzymatic activity. Research interest in FBXW9 stems from its potential involvement in cellular processes such as cell cycle regulation, stress responses, and signaling pathways, though direct mechanistic evidence is limited. Dysregulation of F-box proteins is often linked to cancer, neurodegenerative disorders, and metabolic diseases, positioning FBXW9 as a candidate for exploring novel therapeutic targets.
Current studies focus on identifying its physiological substrates, mapping binding partners, and elucidating its tissue-specific functions. Challenges include resolving its 3D structure and clarifying regulatory post-translational modifications. Recombinant FBXW9 serves as a key tool for these investigations, bridging gaps in understanding the complexity of ubiquitin-dependent proteolysis in health and disease.
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