| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXW8 |
| Uniprot No | Q8N3Y1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598aa |
| 氨基酸序列 | MDDYSLDEFR RRWQEELAQA QAPKKRRRPE AAERRARRPE VGSGRGEQAS GDPALAQRLL EGAGRPPAAR ATRAEGQDVA SRSRSPLARE GAGGGEQLVD QLIRDLNEMN DVPFFDIQLP YELAINIFQY LDRKELGRCA QVSKTWKVIA EDEVLWYRLC QQEGHLPDSS ISDYSCWKLI FQECRAKEHM LRTNWKNRKG AVSELEHVPD TVLCDVHSHD GVVIAGYTSG DVRVWDTRTW DYVAPFLESE DEEDEPGMQP NVSFVRINSS LAVAAYEDGF LNIWDLRTGK YPVHRFEHDA RIQALALSQD DATVATASAF DVVMLSPNEE GYWQIAAEFE VPKLVQYLEI VPETRRYPVA VAAAGDLMYL LKAEDSARTL LYAHGPPVTC LDVSANQVAF GVQGLGWVYE GSKILVYSLE AGRRLLKLGN VLRDFTCVNL SDSPPNLMVS GNMDGRVRIH DLRSGNIALS LSAHQLRVSA VQMDDWKIVS GGEEGLVSVW DYRMNQKLWE VYSGHPVQHI SFSSHSLITA NVPYQTVMRN ADLDSFTTHR RHRGLIRAYE FAVDQLAFQS PLPVCRSSCD AMATHYYDLA LAFPYNHV |
| 分子量 | 67.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXW8蛋白的虚构参考文献示例(非真实文献，供参考格式)：
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1. **文献名称**: *FBXW8 regulates mTOR signaling through targeting DEPTOR for ubiquitination*
**作者**: Li et al.
**摘要**: 本研究报道了重组人FBXW8蛋白通过SCF复合体介导DEPTOR的泛素化降解，负调控mTORC1信号通路。实验采用HEK293细胞表达重组FBXW8.证明其对细胞增殖和代谢的调控作用。
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2. **文献名称**: *Expression and functional characterization of recombinant human FBXW8 in E. coli*
**作者**: Zhang & Wang
**摘要**: 作者优化了人FBXW8蛋白在大肠杆菌中的重组表达与纯化方案，并验证其与Skp1蛋白的相互作用，为后续泛素连接酶活性研究提供工具。
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3. **文献名称**: *FBXW8 interacts with Cyclin D1 to promote cell cycle progression*
**作者**: Gupta et al.
**摘要**: 通过免疫共沉淀实验发现重组FBXW8与Cyclin D1结合，调控其稳定性并影响G1/S期转换，提示其在癌症中的潜在功能。
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4. **文献名称**: *Structural insights into FBXW8-substrate recognition by cryo-EM*
**作者**: Chen et al.
**摘要**: 利用冷冻电镜解析了重组FBXW8-Skp1复合体的三维结构，揭示了其底物结合域的关键氨基酸残基，为靶向设计提供依据。
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注：如需真实文献，建议在PubMed或Web of Science中搜索关键词“FBXW8”、“recombinant FBXW8”。
**Background of Recombinant Human FBXW8 Protein**
FBXW8 (F-box/WD repeat-containing protein 8) is a member of the F-box protein family, characterized by an F-box domain that facilitates interactions with SKP1. a core component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex plays a critical role in ubiquitin-mediated proteasomal degradation, targeting specific substrates for post-translational regulation. FBXW8 contains WD40 repeats, which are essential for substrate recognition and binding.
Recombinant human FBXW8 protein is engineered via molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality. Its production enables detailed studies on molecular mechanisms underlying substrate specificity, interactions within the SCF complex, and roles in cellular processes such as cell cycle regulation, DNA damage response, and apoptosis. Dysregulation of FBXW8 has been implicated in diseases, including certain cancers, where altered ubiquitination pathways contribute to tumorigenesis.
Research on recombinant FBXW8 aids in elucidating its physiological and pathological functions, providing insights into targeted therapeutic strategies. It also serves as a tool for *in vitro* binding assays, structural studies, and screening for modulators of ubiquitin ligase activity. Despite progress, full substrate profiles and regulatory networks of FBXW8 remain under investigation, highlighting the importance of continued exploration.
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