| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC23 |
| Uniprot No | Q8N300 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-66aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEFMDPPA RKEKTKVKES VSRVEKAKQK SAQQELKQRQ RAEIYALNRV MTELEQQQFD EFCKQMQPPG E |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC23重组蛋白的示例参考文献(注：以下文献为示例性虚构内容，实际文献请通过学术数据库检索获取)：
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1. **文献名称**：*Structural characterization of recombinant CCDC23 and its role in cilia assembly*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化了CCDC23重组蛋白，利用X射线晶体学解析其三维结构，发现其卷曲螺旋结构域在纤毛形成中可能参与微管蛋白的稳定化过程。
2. **文献名称**：*Functional analysis of CCDC23 in mitotic spindle organization using recombinant protein mutants*
**作者**：Zhang L, et al.
**摘要**：通过构建CCDC23重组蛋白的截短体和点突变体，发现其C端结构域对细胞分裂中纺锤体定位至关重要，并证明其与DYNC1H1马达蛋白的相互作用依赖磷酸化修饰。
3. **文献名称**：*CCDC23 interacts with microtubule-associated proteins: Evidence from yeast two-hybrid and recombinant pull-down assays*
**作者**：Wang H, et al.
**摘要**：利用重组CCDC23蛋白进行体外结合实验，筛选到与MAP4和EB1的相互作用，提示其在细胞骨架动态调控中的潜在功能。
4. **文献名称**：*Dysregulation of CCDC23 expression in neurodegenerative disorders: Insights from recombinant protein-based models*
**作者**：Brown K, et al.
**摘要**：通过过表达CCDC23重组蛋白的神经元模型，发现其异常表达导致线粒体运输障碍，可能与阿尔茨海默病中轴突退行性变相关。
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**建议**：实际研究中，建议通过PubMed、Web of Science等平台，以“CCDC23 recombinant protein”“CCDC23 function”为关键词检索最新文献，重点关注其在纤毛、细胞分裂或疾病机制中的研究。
**Background of CCDC23 Recombinant Protein**
CCDC23 (Coiled-Coil Domain-Containing Protein 23) is a member of the CCDC family, characterized by conserved coiled-coil domains that mediate protein-protein interactions and structural organization within cells. While its precise biological function remains under investigation, CCDC23 is hypothesized to play roles in cellular processes such as cilia formation, intracellular transport, or cell cycle regulation, based on homology with related proteins and limited experimental data.
Genomic studies link CCDC23 to ciliopathies, a group of disorders caused by dysfunctional cilia, suggesting its potential involvement in ciliary assembly or maintenance. CCDC23 is expressed ubiquitously but shows higher activity in tissues reliant on cilia, such as the respiratory tract, kidneys, and brain. Structural predictions indicate that its coiled-coil domains may facilitate interactions with microtubules or other cytoskeletal components, aligning with its suspected role in cellular architecture.
Recombinant CCDC23 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purification tags (e.g., His-tag) are often incorporated for affinity-based isolation. This recombinant tool aids in elucidating CCDC23's interactome, structural features, and biochemical properties. Researchers employ it in assays such as pull-down experiments, antibody generation, or *in vitro* binding studies to map its partnerships with signaling molecules or cytoskeletal regulators.
Despite progress, gaps persist in understanding CCDC23's mechanistic contributions to health and disease. Current research focuses on validating its association with ciliopathies, cancer (where dysregulated CCDC expression is observed), and neurodegenerative conditions. The availability of recombinant CCDC23 accelerates these efforts, offering a platform to explore its therapeutic relevance or utility as a biomarker. Further studies are needed to define its precise cellular roles and pathological implications.
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