| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ10324 |
| Uniprot No | Q75LH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MSPRVGPVTVARIPASCVHRAGDSGLNLLAPGFPHRWAGRPGEGGSFSCGHYGHPLSPAEDVLESYENPPPIVLPSDGFQVDLEANCLDDSIYQHLLYVRHFLWGLRSRASPGSPGRPGSGASQPVCPEGMHHVVLDGHLEAPSCPLAPRDPGPAAREVAPERTLPLRGAPWAQAPPGRQPGRGGSQAGPPHTDSSCLLTPPSTPLGPEPGDPDWPESGGPCGKALPERQRNGPSGLRGAAPEGDSAALAEESPPAPSSRSSSTEDFCYVFTVELERGPSGLGMGLIDGMHTHLGAPGLYIQTLLPGSPAAADGRLSLGDRILEVNGSSLLGLGYLRAVDLIRHGGKKMRFLVAKSDVETAKKIHFRTPPL |
| 分子量 | 66.55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ10324蛋白的模拟参考文献示例(因该蛋白研究较少，实际文献可能需要进一步检索验证)：
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1. **文献名称**：**Cloning and Functional Characterization of Human FLJ10324 Protein**
**作者**：Zhang Y et al.
**摘要**：该研究报道了FLJ10324基因的克隆及其重组蛋白在大肠杆菌中的表达与纯化，发现该蛋白在体外细胞模型中可能通过调节MAPK信号通路影响细胞增殖。
2. **文献名称**：**FLJ10324 as a Potential Biomarker in Breast Cancer Prognosis**
**作者**：Wang L et al.
**摘要**：通过免疫组化分析发现，FLJ10324蛋白在乳腺癌组织中高表达，并与患者不良预后相关，提示其可能作为肿瘤治疗的潜在靶点。
3. **文献名称**：**Structural Insights into FLJ10324 Protein via Bioinformatics Analysis**
**作者**：Chen J et al.
**摘要**：利用生物信息学方法预测FLJ10324蛋白的三维结构及功能域，揭示其可能参与DNA损伤修复，并通过酵母双杂交实验验证其与BRCA1的相互作用。
4. **文献名称**：**FLJ10324 Regulates Autophagy in Hepatocellular Carcinoma Cells**
**作者**：Kim S et al.
**摘要**：研究表明，重组FLJ10324蛋白可通过抑制mTOR通路激活肝癌细胞自噬，为肝细胞癌的分子机制研究提供了新方向。
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**注意**：以上为模拟文献，具体研究需通过PubMed、Web of Science等学术平台检索确认。若检索结果有限，可尝试扩展关键词(如基因别名**C1orf123**或功能描述)。
**Background of Recombinant Human FLJ10324 Protein**
Recombinant human FLJ10324 protein is a synthetic form of the protein encoded by the FLJ10324 gene (currently annotated under alternative designations, such as C1orf123 or CCDC183. depending on updates in genomic databases). This gene is located on chromosome 1 and is classified as a poorly characterized protein, with limited functional information available in current literature. FLJ10324 is predicted to be a cytoplasmic or secreted protein, potentially involved in cellular processes such as signal transduction or metabolic regulation, though its exact biological role remains under investigation.
The recombinant version is typically produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to enable large-scale purification and functional studies. Structural analyses suggest conserved domains indicative of potential enzymatic or ligand-binding activities. Interest in FLJ10324 stems from its differential expression patterns observed in certain cancers, neurological disorders, or metabolic diseases, implicating its possible role as a biomarker or therapeutic target.
Current research focuses on elucidating its interaction partners, post-translational modifications, and role in disease pathways. Recombinant FLJ10324 protein is critical for generating antibodies, conducting *in vitro* assays, and validating *in silico* predictions. Despite its enigmatic nature, further characterization of this protein may uncover novel mechanisms in cellular physiology or pathology, bridging gaps in understanding human proteome complexity.
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