| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FMNL2 |
| Uniprot No | Q96PY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | MDLTKREYTMHDHNTLLKEFILNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKAYKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQELIAELRRRQVKDNRHVYEGKDGAIEDIITALKKNNITKFPNVHSRVRISSSTPVVEDTQS |
| 分子量 | 45.32 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FMNL2蛋白的参考文献示例(注：以下文献为示例性内容，实际引用需核对真实数据库)：
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1. **文献名称**：*Formin-like 2 (FMNL2) promotes actin polymerization and filopodia formation via its FH2 domain*
**作者**：Block J. et al.
**摘要**：该研究利用重组人FMNL2蛋白进行体外肌动蛋白聚合实验，发现其FH2结构域可直接结合肌动蛋白单体，促进微丝延伸，揭示FMNL2在细胞伪足形成中的关键作用。
2. **文献名称**：*FMNL2 interacts with Rho GTPases to orchestrate invasive cell migration in triple-negative breast cancer*
**作者**：Li H. et al.
**摘要**：通过重组FMNL2蛋白的体外结合实验和癌细胞模型，证明FMNL2与RhoA/C协同作用，调控侵袭性伪足(invadopodia)动态，增强乳腺癌细胞的迁移和转移能力。
3. **文献名称**：*Structural and functional characterization of the autoinhibitory mechanism of FMNL2*
**作者**：Yang X. et al.
**摘要**：采用重组FMNL2蛋白的晶体结构分析，阐明其N端结构域通过分子内相互作用抑制FH2结构域活性，并发现Rho信号通路可解除自抑制状态，激活肌动蛋白组装功能。
4. **文献名称**：*FMNL2 drives extracellular matrix degradation by regulating MMP secretion in macrophages*
**作者**：Chen R. et al.
**摘要**：研究利用重组FMNL2蛋白及基因敲除模型，发现FMNL2通过调控巨噬细胞中基质金属蛋白酶(MMP)的分泌，影响细胞外基质重塑，揭示其在炎症和肿瘤微环境中的作用。
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**注意**：以上文献信息为示例性质，实际研究中需通过PubMed、Google Scholar等平台验证文献真实性并获取完整信息。
FMNL2 (Formin-like protein 2) is a member of the formin family, a group of actin-binding proteins that regulate cytoskeleton dynamics. It plays critical roles in cell migration, adhesion, and cytokinesis by modulating actin polymerization and microtubule organization. The human FMNL2 gene encodes a multi-domain protein containing a Formin Homology 2 (FH2) domain essential for its actin-nucleating activity, along with regulatory regions like the GTPase-binding domain (GBD) and diaphanous inhibitory domain (DID), which enable interaction with Rho-family GTPases for spatiotemporal control of cytoskeletal remodeling.
Recombinant human FMNL2 protein is typically produced using expression systems like *E. coli* or mammalian cells, allowing researchers to study its biochemical properties, structure-function relationships, and interactions with signaling molecules. Studies have linked FMNL2 to pathological processes, including cancer metastasis, immune cell trafficking, and neuronal development. Its overexpression in certain cancers correlates with enhanced invasiveness, while in other contexts, it may suppress tumor growth, suggesting context-dependent roles. Research on recombinant FMNL2 has also highlighted its involvement in membrane protrusion formation (e.g., filopodia, lamellipodia) and intracellular vesicle transport. Current investigations focus on targeting FMNL2-regulated pathways for therapeutic intervention in diseases involving aberrant cell motility or cytoskeletal dysregulation.
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