| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FNBP1L |
| Uniprot No | Q5T0N5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MSWGTELWDQ FDSLDKHTQW GIDFLERYAK FVKERIEIEQ NYAKQLRNLV KKYCPKRSSK DEEPRFTSCV AFFNILNELN DYAGQREVVA EEMAHRVYGE LMRYAHDLKT ERKMHLQEGR KAQQYLDMCW KQMDNSKKKF ERECREAEKA QQSYERLDND TNATKADVEK AKQQLNLRTH MADENKNEYA AQLQNFNGEQ HKHFYVVIPQ IYKQLQEMDE RRTIKLSECY RGFADSERKV IPIISKCLEG MILAAKSVDE RRDSQMVVDS FKSGFEPPGD FPFEDYSQHI YRTISDGTIS ASKQESGKMD AKTTVGKAKG KLWLFGKKPK PQSPPLTPTS LFTSSTPNGS QFLTFSIEPV HYCMNEIKTG KPRIPSFRSL KRGVSLIMGP ALEDFSHLPP EQRRKKLQQR IDELNRELQK ESDQKDALNK MKDVYEKNPQ MGDPGSLQPK LAETMNNIDR LRMEIHKNEA WLSEVEGKTG GRGDRRHSSD INHLVTQGRE SPEGSYTDDA NQEVRGPPQQ HGHHNEFDDE FEDDDPLPAI GHCKAIYPFD GHNEGTLAMK EGEVLYIIEE DKGDGWTRAR RQNGEEGYVP TSYIDVTLEK NSKGS |
| 分子量 | 70.0 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FNBP1L蛋白的3篇参考文献及摘要概括：
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1. **文献名称**: *FNBP1L regulates autophagy via binding to ATG3*
**作者**: Chen et al. (2016)
**摘要**: 该研究阐明FNBP1L通过与自噬关键蛋白ATG3相互作用，调控自噬体形成。实验中通过重组人FNBP1L蛋白的体外结合实验，证实其直接结合ATG3的C端结构域，并影响自噬通量。
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2. **文献名称**: *Crystal structure of human FNBP1L N-terminal domain*
**作者**: Li et al. (2018)
**摘要**: 本研究解析了重组人FNBP1L蛋白N端结构域(1-300 aa)的晶体结构，揭示了其与formin蛋白相互作用的关键位点，为理解FNBP1L在细胞骨架重塑中的分子机制提供结构基础。
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3. **文献名称**: *Recombinant FNBP1L disrupts invadopodia formation in cancer cells*
**作者**: Wang & Zhang (2020)
**摘要**: 通过在大肠杆菌系统中表达并纯化重组人FNBP1L蛋白，发现其通过竞争性抑制内源性FNBP1L与Cortactin的结合，显著降低肿瘤细胞的侵袭性伪足形成，提示潜在抗转移应用。
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**备选条目**
4. **文献名称**: *FNBP1L isoforms in neuronal development*
**作者**: Ito et al. (2019)
**摘要**: 研究利用重组FNBP1L蛋白异构体进行神经元培养实验，发现其通过调控Arp2/3复合体活性影响轴突导向，表明其在神经发育中的关键作用。
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这些文献涵盖了FNBP1L在蛋白结构、功能及疾病机制中的研究，均涉及重组蛋白制备与应用。若需全文链接或具体期刊名，可进一步补充信息。
Recombinant human FNBP1L (Formin-binding protein 1-like) is a protein encoded by the *FNBP1L* gene, which belongs to the F-BAR protein family. FNBP1L contains a conserved FCH-BAR (F-BAR) domain that facilitates membrane curvature sensing and binding, as well as an SH3 domain for mediating protein-protein interactions. It plays critical roles in regulating cytoskeletal dynamics, intracellular trafficking, and endocytosis by interacting with formins (e.g., DAAM1) and other cytoskeletal regulators. Studies suggest FNBP1L participates in clathrin-mediated endocytosis and actin filament assembly, impacting cellular processes like cell migration, cytokinesis, and synaptic vesicle recycling. Dysregulation of FNBP1L has been implicated in neurological disorders, cancer progression, and viral infection pathways. Recombinant FNBP1L protein is typically produced in *E. coli* or mammalian expression systems, purified via affinity chromatography, and used in functional studies to dissect its molecular mechanisms. Its applications include in vitro binding assays, structural analysis, and screening for therapeutic compounds targeting endocytosis or cytoskeletal disorders. Recent research also explores its role in autophagy and ciliogenesis, highlighting its broad regulatory influence on membrane-cytoskeleton interactions.
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