| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FMNL3 |
| Uniprot No | Q8IVF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347aa |
| 氨基酸序列 | MRFLPTEAEVKLLRQYERERQPLEELAAEDRFMLLFSKVERLTQRMAGMAFLGNFQDNLQMLTPQLNAIIAASASVKSSQKLKQMLEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKMTLLHFIALTVKEKYPDLANFWHELHFVEKAAAVSLENVLLDVKELGRGMELIRRECSIHDNSVLRNFLSTNEGKLDKLQRDAKTAEEAYNAVVRYFGESPKTTPPSVFFPVFVRFIRSYKEAEQENEARKKQEEVMREKQLAQEAKKLDAKTPSQRNKWQQQELIAELRRRQAKEHRPVYEGKDGTIEDIITVLKSVPFTARTAKRGSRFFCDAAHHDESNC |
| 分子量 | 66.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人FMNL3蛋白相关的3篇参考文献的简要列举：
1. **文献名称**: *FMNL3 regulates glioblastoma progression and epithelial-mesenchymal transition via cytoskeletal dynamics*
**作者**: Li R et al.
**摘要**: 研究了FMNL3通过调控细胞骨架动态驱动胶质母细胞瘤侵袭的机制，利用重组FMNL3蛋白验证其与Arp2/3复合体的相互作用，揭示其在肿瘤迁移中的作用。
2. **文献名称**: *Structural and functional insights into the interaction of formin FMNL3 with actin*
**作者**: Wang C et al.
**摘要**: 通过重组FMNL3蛋白的体外表达，解析其formin结构域结合肌动蛋白的分子机制，表明FMNL3通过促进线性微丝组装影响细胞运动。
3. **文献名称**: *FMNL3 promotes colon cancer metastasis by modulating Wnt/β-catenin signaling*
**作者**: Zhang Y et al.
**摘要**: 利用重组FMNL3蛋白进行功能验证，发现其通过增强β-catenin核转位促进结肠癌细胞迁移，为FMNL3在癌症转移中的信号通路提供了证据。
(注：以上文献为虚构示例，实际研究中建议通过PubMed等数据库搜索具体论文。)
Formin-like 3 (FMNL3), a member of the Formin protein family, is an actin-nucleating factor involved in cytoskeletal remodeling and regulation of cell motility. It plays critical roles in diverse cellular processes, including cell adhesion, migration, and invasion, by promoting the polymerization of linear actin filaments. FMNL3 is particularly notable for its involvement in cancer progression, where its overexpression has been linked to enhanced tumor cell invasiveness and metastasis in cancers such as colorectal, gastric, and breast cancer. It interacts with Rho GTPases (e.g., Cdc42) and other signaling molecules to coordinate membrane protrusion and cytoskeletal dynamics during directional cell movement.
Recombinant human FMNL3 protein, typically produced in *E. coli* or mammalian expression systems, enables in vitro studies to dissect its biochemical properties, structural domains (e.g., FH1. FH2. and GTPase-binding regions), and functional interactions. Researchers utilize this protein to explore mechanisms underlying FMNL3-mediated actin remodeling, its role in epithelial-mesenchymal transition (EMT), and its potential as a therapeutic target. Mutant variants or truncated forms of recombinant FMNL3 are often engineered to investigate domain-specific activities or to inhibit pathological functions. Studies using this protein have also contributed to understanding its non-cancer-related roles, such as in immune cell trafficking and cytokinesis. Its application spans basic research, drug screening, and biomarker validation in oncology.
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