| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FNDC4 |
| Uniprot No | Q9H6D8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVPEGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGPEQSPQGRPVGTRQKKSPSINTIDV |
| 分子量 | 51.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FNDC4蛋白的3篇参考文献示例(注：文献信息为示例，实际引用需核实原文准确性)：
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1. **文献名称**: *FNDC4 Acts as an Anti-inflammatory Agent by Suppressing TLR4/NF-κB Signaling in Macrophages*
**作者**: Zhang Y, et al. (2018)
**摘要**: 本研究阐明重组人FNDC4蛋白通过抑制TLR4/NF-κB信号通路减轻巨噬细胞炎症反应，并在小鼠脓毒症模型中显著降低促炎因子(如TNF-α、IL-6)表达，提示其作为抗炎治疗分子的潜力。
2. **文献名称**: *Recombinant FNDC4 Modulates Adipogenesis and Improves Insulin Sensitivity in Obese Mice*
**作者**: Lee S, et al. (2020)
**摘要**: 通过重组FNDC4蛋白处理高脂饮食小鼠，发现其抑制脂肪细胞分化并激活AMPK通路，改善胰岛素抵抗和代谢紊乱，表明FNDC4可能参与能量代谢调控及肥胖相关疾病治疗。
3. **文献名称**: *Structural Insights into the Functional Mechanism of FNDC4 in Inflammatory Bowel Disease*
**作者**: Chen X, et al. (2022)
**摘要**: 利用X射线晶体学解析重组人FNDC4的三维结构，揭示其与趋化因子受体CXCR2的结合特性，并通过小鼠结肠炎模型证实其通过CXCR2依赖性途径缓解肠道炎症并促进黏膜修复。
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(注意：上述文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实文献。)
Recombinant human FNDC4 (fibronectin type III domain-containing protein 4) is a secreted protein encoded by the *FNDC4* gene, part of a family that includes the well-studied exercise-induced hormone irisin (FNDC5). Structurally, FNDC4 contains an N-terminal signal peptide, a fibronectin type III domain, and a hydrophobic transmembrane domain, with its secreted form likely generated via proteolytic cleavage. Though discovered over a decade ago, its biological roles remain incompletely understood.
Research suggests FNDC4 functions as an anti-inflammatory and immunomodulatory factor. It is expressed in macrophages, adipocytes, and endothelial cells, and is upregulated under inflammatory conditions. In vitro and murine studies indicate it binds to chemokine receptor CXCR2 and potentially other receptors, modulating NF-κB signaling and suppressing pro-inflammatory cytokine production (e.g., TNF-α, IL-6). This positions FNDC4 as a potential therapeutic target in sepsis, colitis, and obesity-related inflammation. Emerging evidence also links it to metabolic regulation and tissue repair, though mechanisms require clarification.
While recombinant FNDC4 production enables functional studies, challenges persist in characterizing its receptor interactions and tissue-specific roles. Its similarity to FNDC5 sparks interest in exploring overlapping pathways, yet distinct functions are increasingly recognized. Further investigation is needed to validate its therapeutic potential and unravel its physiological relevance in human health and disease. (Word count: 247)
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