| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXC2 |
| Uniprot No | Q99958 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-501aa |
| 氨基酸序列 | MQARYSVSDPNALGVVPYLSEQNYYRAAGSYGGMASPMGVYSGHPEQYSAGMGRSYAPYHHHQPAAPKDLVKPPYSYIALITMAIQNAPEKKITLNGIYQFIMDRFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENGSFLRRRRRFKKKDVSKEKEERAHLKEPPPAASKGAPATPHLADAPKEAEKKVVIKSEAASPALPVITKVETLSPESALQGSPRSAASTPAGSPDGSLPEHHAAAPNGLPGFSVENIMTLRTSPPGGELSPGAGRAGLVVPPLALPYAAAPPAAYGQPCAQGLEAGAAGGYQCSMRAMSLYTGAERPAHMCVPPALDEALSDHPSGPTSPLSALNLAAGQEGALAATGHHHQHHGHHHPQAPPPPPAPQPQPTPQPGAAAAQAASWYLNHSGDLNHLPGHTFAAQQQTFPNVREMFNSHRLGIENSTLGESQVSGNASCQLPYRSTPPLYRHAAPYSYDCTKY |
| 分子量 | 82.06 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人FOXC2蛋白相关的3篇参考文献示例(注：内容为示例性虚构，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and purification of recombinant human FOXC2 in Escherichia coli for functional analysis*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化重组人FOXC2蛋白，验证其通过叉头结构域特异性结合靶DNA序列的能力，为后续研究其转录调控功能提供工具。
2. **文献名称**：*FOXC2 promotes epithelial-mesenchymal transition in breast cancer via regulation of Slug*
**作者**：Lee H, et al.
**摘要**：通过体外细胞实验发现，重组人FOXC2蛋白过表达可激活Slug基因，诱导乳腺癌细胞发生上皮-间质转化(EMT)，增强迁移和侵袭能力。
3. **文献名称**：*Structural and functional analysis of the C-terminal domain of FOXC2*
**作者**：Zhang Y, et al.
**摘要**：解析FOXC2蛋白的C端结构域，发现其参与蛋白稳定性调控，并通过截断体实验证明该区域缺失会显著降低其对下游靶基因的转录激活活性。
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如需真实文献，建议通过PubMed、Web of Science等平台检索关键词“recombinant FOXC2 protein”或“FOXC2 function”。
Forkhead box protein C2 (FOXC2) is a member of the Forkhead family of transcription factors, characterized by a conserved DNA-binding domain known as the "Forkhead box." It plays crucial roles in embryonic development, cell differentiation, and tissue homeostasis. FOXC2 is particularly noted for its involvement in regulating lymphatic and cardiovascular system formation, adipocyte metabolism, and epithelial-mesenchymal transition (EMT). Mutations in the FOXC2 gene are linked to hereditary lymphedema-distichiasis syndrome, a condition characterized by abnormal lymphatic function and accessory eyelash growth. Recombinant human FOXC2 protein is produced using genetic engineering techniques, often in bacterial or mammalian expression systems, to enable functional studies. This purified protein retains the transcription factor's ability to bind specific DNA sequences and regulate downstream gene expression. Researchers utilize recombinant FOXC2 to investigate its role in cancer metastasis, fibrosis, metabolic disorders, and lymphatic-related pathologies. Its study provides insights into molecular mechanisms underlying developmental defects and potential therapeutic targets for diseases involving abnormal cell proliferation or differentiation. Current research also explores FOXC2's dual role as both a tumor suppressor and promoter in different cancer contexts.
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